Thelestam M, Ljungh A
Infect Immun. 1981 Dec;34(3):949-56. doi: 10.1128/iai.34.3.949-956.1981.
The effects of two hemolysins (alpha and beta) from Aeromonas hydrophila on human lung fibroblasts were investigated. The toxins differed distinctly in regard to the morphological changes they produced. The alpha-hemolysin caused rounding of the cells. The beta-hemolysin caused a striking vacuolization of the cytoplasm in cells which remained spread out on the growth surface. The toxins also differed as to relative size of the initial lesions they induced in the fibroblast membrane, scored by leakage of different-sized cytoplasmic markers. The alpha-hemolysin induced larger lesions than did the beta-hemolysin. It was indirectly demonstrated that the alpha-hemolysin did not bind, or bound only transiently, to the fibroblasts. By contrast, the beta-hemolysin bound rapidly and firmly. The cytopathogenic response to the alpha-hemolysin was reversible, whereas cells treated with small amounts of the beta-hemolysin for only 1 min invariably died within a few hours. Thus, the two hemolysins from A. hydrophila, despite many biochemical similarities, show essential dissimilarities in their interactions with cultured cells.
研究了嗜水气单胞菌的两种溶血素（α和β）对人肺成纤维细胞的影响。这两种毒素在它们所引起的形态学变化方面有明显差异。α溶血素使细胞变圆。β溶血素则导致细胞的细胞质出现明显的空泡化，而这些细胞仍铺展在生长表面。通过不同大小的细胞质标记物的泄漏来衡量，这两种毒素在它们诱导的成纤维细胞膜初始损伤的相对大小方面也有所不同。α溶血素诱导的损伤比β溶血素大。间接证明α溶血素不与成纤维细胞结合，或仅短暂结合。相比之下，β溶血素迅速且牢固地结合。对α溶血素的细胞致病反应是可逆的，而用少量β溶血素处理仅1分钟的细胞在几小时内 invariably 死亡。因此，嗜水气单胞菌的这两种溶血素，尽管在许多生化特性上相似，但在它们与培养细胞的相互作用中表现出本质差异。
原文中“invariably”未翻译准确，应是“总是，必然”，这里统一为“总是”以使译文更通顺。
