Truncating the Structure of Lipopolysaccharide in Can Effectively Improve Poly-3-hydroxybutyrate Production.

Poly-3-hydroxybutyrate is an environmentally friendly polymer with many promising applications and can be produced in cells after overexpressing the heterologous gene cluster . In this study, we found that truncating the structure of lipopolysaccharide in can effectively enhance poly-3-hydroxybutyrate production. mutant strains WJW00, WJD00, and WJJ00 were constructed by deleting from strain W3110, DH5α, and JM109, respectively. Compared to the controls W3110/pDXW-8-, DH5a/pDXW-8-, and JM109/pDXW-8-, the yield of poly-3-hydroxybutyrate in WJW00/pDXW-8-, WJD00/pDXW-8-, and WJJ00/pDXW-8- cells increased by 200%, 81.5%, and 75.6%, respectively, and the conversion rate of glucose to poly-3-hydroxybutyrate was increased by ∼250%. Further analysis revealed that LPS truncation in rebalanced carbon and nitrogen metabolism, increased the levels of acetyl-CoA, γ-aminobutyric acid, NADPH, NADH, and ATP, and decreased the levels of organic acids and flagella, resulting in the high ratio of carbon to nitrogen. These metabolic changes in these mutants led to the significant increase of poly-3-hydroxybutyrate production.