Department of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Pécs, Hungary.
Biocentrum Ltd., Gyongyosoroszi, Hungary.
PLoS One. 2020 Apr 17;15(4):e0231864. doi: 10.1371/journal.pone.0231864. eCollection 2020.
Metagenomics is a helpful tool for the analysis of unculturable organisms and viruses. Viruses that target bacteria and archaea play important roles in the microbial diversity of various ecosystems. Here we show that Methanosarcina virus MV (MetMV), the second Methanosarcina sp. virus with a completely determined genome, is characteristic of hydrocarbon pollution in environmental (soil and water) samples. It was highly abundant in Hungarian hydrocarbon polluted samples and its genome was also present in the NCBI SRA database containing reads from hydrocarbon polluted samples collected in Canada, indicating the stability of its niche and the marker feature of this virus. MetMV, as the only currently identified marker virus for pollution in environmental samples, could contribute to the understanding of the complicated network of prokaryotes and their viruses driving the decomposition of environmental pollutants.
宏基因组学是分析不可培养生物和病毒的有用工具。针对细菌和古菌的病毒在各种生态系统的微生物多样性中发挥着重要作用。在这里，我们展示了 Methanosarcina 病毒 MV（MetMV），这是第二个具有完全确定基因组的 Methanosarcina sp. 病毒，它是环境（土壤和水）样本中碳氢化合物污染的特征。它在匈牙利碳氢化合物污染的样本中含量很高，其基因组也存在于 NCBI SRA 数据库中，其中包含了从加拿大碳氢化合物污染样本中采集的reads，这表明了它的生态位的稳定性和该病毒的标记特征。MetMV 作为目前唯一鉴定出的环境样本污染标记病毒，可以帮助我们理解驱动环境污染物分解的复杂原核生物及其病毒网络。
