Laboratório de Imunologia das Doenças Infecciosas e Crônicas, Universidade José do Rosário Vellano, Alfenas, Brazil.
Departamento de Microbiologia e Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil.
Am J Reprod Immunol. 2020 Jul;84(1):e13247. doi: 10.1111/aji.13247. Epub 2020 May 10.
Gut dysbiosis is caused by several factors, including the use of antibiotics. Since intestinal dysbiosis is associated with a wide range of immunopathological and reproductive conditions, the main goal of this study was to evaluate amoxicillin-induced gut dysbiosis and its influence on the oestrous cycle in mice.
Mice were treated with amoxicillin or PBS, and faecal microbiota was evaluated by 16S rDNA metagenomic sequencing. The oestrous cycle was evaluated by vaginal cytology, vaginal opening and flow cytometry. After the induction of gut dysbiosis, the ovaries and the caecum were analysed to differential expression of IL-1β and IL-10 genes and histological analysis.
Amoxicillin-treated mice presented differing bacterial groups in the faecal microbiota when compared to the PBS-treated group indicating that amoxicillin treatment-induced gut dysbiosis and they gained weight. The vaginal cytology analysis showed that amoxicillin-induced gut dysbiosis decreased the number of cells but increased the relative number of leucocytes and altered the oestrous cycle. IL-1β was shown to be upregulated in the caecum and in the ovary of the dysbiotic mice. On the other hand, IL-10 expression was shown to be diminished in both organs of the dysbiotic mice. The oocyte area from dysbiotic group presented lower than non-dysbiotic mice with increasing thickness of the pellucid zone. The follicular teak from dysbiotic mice showed lower thickness than non-dysbiotic mice.
The results indicate that amoxicillin induces gut dysbiosis and influences the oestrous cycle and the inflammatory status of the ovary and the caecum.
肠道菌群失调是由多种因素引起的，包括使用抗生素。由于肠道菌群失调与广泛的免疫病理和生殖状况有关，本研究的主要目的是评估阿莫西林诱导的肠道菌群失调及其对小鼠发情周期的影响。
用阿莫西林或 PBS 处理小鼠，通过 16S rDNA 宏基因组测序评估粪便微生物群。通过阴道细胞学、阴道开口和流式细胞术评估发情周期。在诱导肠道菌群失调后，分析卵巢和盲肠中 IL-1β 和 IL-10 基因的差异表达和组织学分析。
与 PBS 处理组相比，阿莫西林处理组的粪便微生物群中存在不同的细菌群，表明阿莫西林治疗诱导了肠道菌群失调和体重增加。阴道细胞学分析表明，阿莫西林诱导的肠道菌群失调减少了细胞数量，但增加了白细胞的相对数量并改变了发情周期。IL-1β 在肠道失调小鼠的盲肠和卵巢中均上调。另一方面，IL-10 的表达在失调小鼠的两个器官中均减少。失调组的卵母细胞面积小于非失调组，透明带厚度增加。失调组的滤泡树突状细胞厚度小于非失调组。
结果表明，阿莫西林诱导肠道菌群失调，影响发情周期以及卵巢和盲肠的炎症状态。
