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采用基于变角动态磁化传递（VDMP）CEST MRI 技术评估细胞移植对实验性自身免疫性脑脊髓炎弥漫性损伤的抑制作用。

Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI.

机构信息

The Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, United States of America; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, United States of America.

The Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, United States of America; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, United States of America.

出版信息

Exp Neurol. 2020 Jul;329:113316. doi: 10.1016/j.expneurol.2020.113316. Epub 2020 Apr 15.

DOI:10.1016/j.expneurol.2020.113316
PMID:
32304749
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7772822/
Abstract

The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.

摘要

细胞疗法的发展和转化受到阻碍，因为无法预测和评估其移植后的疗效。我们使用多发性硬化症（MS）的实验性自身免疫性脑脊髓炎（EAE）小鼠模型，研究了胶质限制定向前体细胞（GRP）移植对 EAE 和 MS 的弥漫性损伤的抑制作用。我们评估了共振变量延迟多脉冲（onVDMP）化学交换饱和转移（CEST）MRI 来可视化这种衰减的潜力。在运动皮层或侧脑室中移植同种异体 GRP 可减轻 EAE 小鼠的瘫痪，并在移植后 5 天在移植部位附近，与未受感染的小鼠相比，减轻了 onVDMP CEST 信号的差异。组织学分析显示，移植的 GRP 与减弱的星形胶质细胞增生共定位。因此，弥散性损伤敏感的 onVDMP CEST MRI 可与常规 MRI 互补，以定位和监测对 GRP 治疗有反应的组织区域。

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本文引用的文献

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Monitoring diffuse injury during disease progression in experimental autoimmune encephalomyelitis with on resonance variable delay multiple pulse (onVDMP) CEST MRI.采用共振可变延迟多重脉冲（onVDMP） CEST MRI 监测实验性自身免疫性脑脊髓炎进展过程中的弥散性损伤。
Neuroimage. 2020 Jan 1;204:116245. doi: 10.1016/j.neuroimage.2019.116245. Epub 2019 Oct 9.
2
Exogenous Neural Precursor Cell Transplantation Results in Structural and Functional Recovery in a Hypoxic-Ischemic Hemiplegic Mouse Model.外源性神经前体细胞移植可促进缺氧缺血性偏瘫小鼠模型的结构和功能恢复。
eNeuro. 2018 Dec 4;5(5). doi: 10.1523/ENEURO.0369-18.2018. eCollection 2018 Sep-Oct.
3
Glutamate-sensitive imaging and evaluation of cognitive impairment in multiple sclerosis.
谷氨酸敏感成像在多发性硬化认知障碍评估中的应用。
Mult Scler. 2019 Oct;25(12):1580-1592. doi: 10.1177/1352458518799583. Epub 2018 Sep 19.
4
GlucoCEST imaging with on-resonance variable delay multiple pulse (onVDMP) MRI.基于共振可变延迟多脉冲（onVDMP）MRI 的 GlucoCEST 成像。
Magn Reson Med. 2019 Jan;81(1):47-56. doi: 10.1002/mrm.27364. Epub 2018 Jul 29.
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CEST, ASL, and magnetization transfer contrast: How similar pulse sequences detect different phenomena.CEST、ASL 和磁化转移对比：不同脉冲序列如何检测不同现象。
Magn Reson Med. 2018 Oct;80(4):1320-1340. doi: 10.1002/mrm.27341. Epub 2018 May 30.
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Brain Region-Dependent Rejection of Neural Precursor Cell Transplants.脑区依赖性对神经前体细胞移植的排斥反应。
Front Mol Neurosci. 2018 Apr 30;11:136. doi: 10.3389/fnmol.2018.00136. eCollection 2018.
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F-VC701-PET and MRI in the in vivo neuroinflammation assessment of a mouse model of multiple sclerosis.F-VC701-PET 和 MRI 用于评估多发性硬化症小鼠模型的体内神经炎症。
J Neuroinflammation. 2018 Feb 5;15(1):33. doi: 10.1186/s12974-017-1044-x.
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