Department of Entomology and Plant Pathology, Cell and Molecular Biology Program, University of Arkansas Systems Division of Agriculture, Fayetteville, AR 72701, USA.
Department of Physics, Cell and Molecular Biology Program, Microelectronics Photonics Program, University of Arkansas, Fayetteville, AR 72701, USA.
Fungal Genet Biol. 2020 Jul;140:103385. doi: 10.1016/j.fgb.2020.103385. Epub 2020 Apr 17.
The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium, which is used to break into plant cells by directed application of enormous turgor force. Appressorium-mediated plant infection requires timely assembly of a higher-order septin ring structure at the base of the appressorium, which is needed to spatially orchestrate appressorium repolarization. Here we use quantitative 4D widefield fluorescence imaging to gain new insight into the spatiotemporal dynamics of septin ring formation, and septin-mediated actin re-organization, during appressorium morphogenesis by M. oryzae. We anticipate that the new knowledge will provide a quantitative framework for dissecting the molecular mechanisms of higher-order septin ring assembly in this devastating plant pathogenic fungus.
稻瘟病菌 Magnaporthe oryzae 分化出一种专门的感染结构，称为附着胞，它通过定向施加巨大膨压来侵入植物细胞。附着胞介导的植物感染需要在附着胞底部及时组装更高阶的隔膜环结构，这对于空间协调附着胞的再极化是必要的。在这里，我们使用定量的 4D 宽场荧光成像，深入了解稻瘟病菌附着胞形态发生过程中隔膜环形成和隔膜介导线粒体重新组织的时空动力学。我们预计，新的知识将为剖析这种破坏性植物病原真菌中更高阶隔膜环组装的分子机制提供一个定量框架。
