State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, PR China.
Bioresour Technol. 2020 Aug;309:123362. doi: 10.1016/j.biortech.2020.123362. Epub 2020 Apr 10.
An efficient biomass and high-value bioactive substances production strategy was developed for unicellular microalgae Porphyridium purpureum. We studied the optimal culture method and metabolites accumulation under different C/N conditions, and further proposed methods to increase the yield under high C/N ratio. The highest biomass reached 16.24 g/L with ASW medium by mixotrophy. High C/N ratio and mediate C/N can significantly promote the synthesis and secretion of polysaccharides, as well as the accumulation of ω-6 PUFAs; however, inhibit the growth, resulting in lower yield. With the significant increase of C/N ratio, protein degradation was accelerated, providing sufficient nitrogen source for efficient accumulation of carbohydrates (1.66 g/L EPS) and PUFAs (231.24 mg/L ARA). Finally, we reduced the growth inhibition, shortened the culture cycle, and doubled the final biomass to 9.34 g/L under nitrogen deficiency condition. Our exploitation of a cost-effective and feasible culture method for red algae is particularly significant.
我们开发了一种高效的生物质和高价值生物活性物质生产策略，用于单细胞微藻紫球藻（Porphyridium purpureum）。我们研究了不同 C/N 条件下的最佳培养方法和代谢产物积累，并进一步提出了在高 C/N 比下提高产量的方法。在混合营养条件下，使用 ASW 培养基可达到最高生物量 16.24 g/L。高 C/N 比和中 C/N 比可显著促进多糖的合成和分泌，以及 ω-6 PUFAs 的积累；然而，这会抑制生长，导致产量较低。随着 C/N 比的显著增加，蛋白质降解加速，为高效积累碳水化合物（1.66 g/L EPS）和 PUFAs（231.24 mg/L ARA）提供了充足的氮源。最后，我们在氮缺乏条件下，通过减少生长抑制、缩短培养周期，将最终生物量提高到 9.34 g/L，实现了产量翻倍。我们对红藻进行了经济高效且可行的培养方法开发，这具有重要意义。
