Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Center for Research in Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), University of Almeria, Agrifood Campus of International Excellence, CeiA3, E-04120 Almeria Spain.
Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Center for Research in Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), University of Almeria, Agrifood Campus of International Excellence, CeiA3, E-04120 Almeria Spain.
J Chromatogr A. 2020 Jul 5;1622:461118. doi: 10.1016/j.chroma.2020.461118. Epub 2020 Apr 13.
A novel application, which combines targeted and non-targeted approaches, using gas chromatography high-resolution mass spectrometry (GCHRMS) for multiresidue analysis of pesticides in fruits and vegetables is proposed; the aim is to add information to that obtained from targeted methods to be used in pesticide residues monitoring laboratories. Extract from fruit samples obtained with QuEChERS method are analysed with two different approaches: a first step in targeted mode in full scan, and a second step in non-targeted mode with HRMS deconvolution. The targeted method was validated for 191 pesticides, in banana, watermelon, pear and strawberry, with recoveries within 70-120%, precision-RSD <20%, linearity between 1-100 µg/kg (5-100 µg/kg in strawberry) and quantification limits (LOQs) of 5 µg/kg. For targeted method, 12 different pesticides in 14 samples were detected. The non-targeted approach allowed the corroboration of all the results obtained through targeted method, in addition other compounds were tentatively identified.
本研究提出了一种新的应用方法，将靶向和非靶向方法相结合，利用气相色谱高分辨率质谱（GCHRMS）对水果和蔬菜中的农药进行多残留分析；目的是为农药残留监测实验室提供靶向方法获得的信息之外的补充信息。使用 QuEChERS 方法从水果样品中提取，采用两种不同的方法进行分析：首先在全扫描靶向模式下进行第一步分析，然后在 HRMS 去卷积的非靶向模式下进行第二步分析。该靶向方法在香蕉、西瓜、梨和草莓中对 191 种农药进行了验证，回收率在 70-120%之间，精密度-RSD<20%，线性范围在 1-100μg/kg（草莓为 5-100μg/kg），定量限（LOQs）为 5μg/kg。通过靶向方法在 14 个样本中检测到 12 种不同的农药。非靶向方法除了可以对靶向方法获得的所有结果进行验证外，还可以对其他化合物进行初步鉴定。
