Araújo Carolina Akiko Sato Cabral, Minervino Antonio Humberto Hamad, Sousa Rejane Santos, Oliveira Francisco Leonardo Costa, Rodrigues Frederico Augusto Mazzocca Lopes, Mori Clara Satsuki, Ortolani Enrico Lippi
Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.
Laboratório de Sanidade Animal, LARSANA, Universidade Federal do Oeste do Pará, UFOPA, Santarem, Brazil.
PeerJ. 2020 Apr 10;8:e8933. doi: 10.7717/peerj.8933. eCollection 2020.
The end of pregnancy is the period with the highest risk of occurrences of ketosis and pregnancy toxemia due to fat mobilization and increasing non-esterified fatty acids in the liver which are converted in ketone bodies, mainly β-hydroxybutyrate acid (BHB). This ketone body may also become elevated in the bloodstream. The present study validates the use of a handheld meter for determining the blood concentration of BHB and ascertaining the predictive value and accuracy of BHB measurements in diagnosing hyperketonaemia in ewes.
A total of 19, non-pregnant, crossbred ewes were subjected to 2 h of intravenous infusion of a saturated BHB solution. Over 6 h of evaluation, 247 blood samples were obtained in 13 sampling moments. The BHB concentration was measured by an enzymatic colorimetric method in an automated biochemical analyzer (gold-standard) and by a handheld meter using an electrochemical enzyme technique.
There was a high correlation between both methods ( = 0.98; < 0.001). Considering the blood BHB concentrations range 0.8-1.6 mmol/L for moderate ketosis the handheld meter presented sensitivity and specificity of 0.98 and 0.81, respectively. For severe ketosis (BHB ≥ 1.6 mmol/L) sensitivity and specificity were 0.99 and 0.75, respectively. Thus, the handheld device can be useful for diagnoses of cases of mild or severe pregnancy toxemia at field conditions.
妊娠末期是酮症和妊娠毒血症发生风险最高的时期，这是由于脂肪动员以及肝脏中非酯化脂肪酸增加，这些脂肪酸会转化为酮体，主要是β-羟基丁酸（BHB）。这种酮体在血液中的浓度也可能升高。本研究验证了使用手持血糖仪测定BHB血液浓度，并确定BHB测量在诊断母羊高酮血症中的预测价值和准确性。
总共19只非妊娠杂交母羊接受了2小时的饱和BHB溶液静脉输注。在6小时的评估过程中，在13个采样时刻采集了247份血样。通过自动生化分析仪中的酶比色法（金标准）和使用电化学酶技术的手持血糖仪测量BHB浓度。
两种方法之间存在高度相关性（ = 0.98； < 0.001）。对于中度酮症，考虑血液BHB浓度范围为0.8 - 1.6 mmol/L，手持血糖仪的灵敏度和特异性分别为0.98和0.81。对于重度酮症（BHB≥1.6 mmol/L），灵敏度和特异性分别为0.99和0.75。因此，该手持设备可用于在现场条件下诊断轻度或重度妊娠毒血症病例。
