Department of Radiation Oncology, Shandong Cancer Hospital affiliated to Shandong University, Jinan, 250117, China.
School of Computer Science and Engineering, Southeast University, Nanjing, China.
Mol Cancer. 2019 Jan 23;18(1):16. doi: 10.1186/s12943-018-0936-4.
Esophageal squamous cell cancer (ESCC) is a high incidence and mortality disease worldwide. However, specificity and sensitivity of its diagnostic and prognostic biomarkers are still unsatisfactory. Recently, circular RNAs (circRNAs) as biomarkers have been studied extensively while the expression profile and clinical significance of circRNAs in ESCC have rarely been studied. We performed circular RNA microarray in 3 pairs of ESCC frozen tumor and non-tumor tissues to identify ESCC-related circRNAs and found 1045 up-regulated and 1032 down-regulated circRNAs among which 6 circRNAs (hsa_circ_0062459, hsa_circ_0076535, hsa_circ_0072215, hsa_circ_0042261, hsa_circ_0001946, and hsa_circ_0043603) displayed consistency with microarray results by qRT-PCR. 3 circRNAs (hsa_circ_0062459, hsa_circ_0001946, and hsa_circ_0043603) were also detected in plasma and 2 of them except hsa_circ_0062459 could be used as diagnostic biomarkers and found in exosome of cell-conditioned culture conditioned media. The AUC, sensitivity and specificity of hsa_circ_0001946 were 0.894, 92, 80%, of hsa_circ_0043603 were 0.836, 64, 92% while a signature combining them were 0.928, 84 and 98%. Hsa_circ_0001946 was confirmed to predict the recurrence, overall survival (OS) and disease-free survival (DFS) in frozen and FFPE tissues, while its overexpression decreased cell proliferation, migration, and invasion.
食管鳞状细胞癌 (ESCC) 是一种在全球范围内发病率和死亡率都很高的疾病。然而，其诊断和预后生物标志物的特异性和敏感性仍然不尽如人意。最近，环状 RNA (circRNA) 作为生物标志物得到了广泛研究，而 circRNA 在 ESCC 中的表达谱和临床意义却很少被研究。我们对 3 对 ESCC 冷冻肿瘤组织和非肿瘤组织进行了环状 RNA 微阵列分析，以鉴定与 ESCC 相关的 circRNA，发现了 1045 个上调和 1032 个下调的 circRNA，其中 6 个 circRNA (hsa_circ_0062459、hsa_circ_0076535、hsa_circ_0072215、hsa_circ_0042261、hsa_circ_0001946 和 hsa_circ_0043603) 通过 qRT-PCR 与微阵列结果一致。3 个 circRNA (hsa_circ_0062459、hsa_circ_0001946 和 hsa_circ_0043603) 在血浆中也有检测到，其中除了 hsa_circ_0062459 之外的 2 个可以作为诊断生物标志物，并且可以在细胞条件培养基的外泌体中发现。hsa_circ_0001946 的 AUC、敏感性和特异性分别为 0.894、92、80%，hsa_circ_0043603 分别为 0.836、64、92%，而结合它们的特征分别为 0.928、84 和 98%。hsa_circ_0001946 被证实可以预测冷冻和 FFPE 组织中的复发、总生存 (OS) 和无病生存 (DFS)，而过表达降低了细胞增殖、迁移和侵袭。
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