Department of Respiratory and Critical Care Medicine, West China Medical School/West China Hospital, Sichuan University, Chengdu, 610041, China.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Mol Cancer. 2019 Sep 4;18(1):134. doi: 10.1186/s12943-019-1061-8.
Emerging evidences demonstrate that circular RNAs (circRNAs) are abnormally expressed in tumors and could serve as prognostic markers for cancers. However, the expression patterns and clinical implications of circRNAs in non-small cell lung cancer (NSCLC) remain obscure. In this study, we profiled circRNA expressions in 10 pairs of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) after ribosomal RNA-depletion and RNase R digestion to enrich circRNAs. Combining five circRNA computational programs, we found that LUAD and LUSC not only share common expression patterns, but also exhibit distinct circRNA expression signatures. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa_circ_0077837 and hsa_circ_0001821 could serve as potential biomarkers for both LUAD and LUSC, while hsa_circ_0001073 and hsa_circ_0001495 could be diagnostic/subtyping marker for LUAD and LUSC, respectively. Therefore, our findings highlight the important diagnostic potential of circRNAs in NSCLC.
越来越多的证据表明，环状 RNA（circRNA）在肿瘤中异常表达，可作为癌症的预后标志物。然而，circRNA 在非小细胞肺癌（NSCLC）中的表达模式和临床意义仍不清楚。在这项研究中，我们通过核糖体 RNA 耗竭和 RNase R 消化对 10 对肺腺癌（LUAD）和鳞状细胞癌（LUSC）进行了 circRNA 表达谱分析，以富集 circRNA。结合五个 circRNA 计算程序，我们发现 LUAD 和 LUSC 不仅共享共同的表达模式，而且还表现出不同的 circRNA 表达特征。此外，受试者工作特征（ROC）曲线分析表明，hsa_circ_0077837 和 hsa_circ_0001821 可作为 LUAD 和 LUSC 的潜在生物标志物，而 hsa_circ_0001073 和 hsa_circ_0001495 可分别作为 LUAD 和 LUSC 的诊断/亚型标志物。因此，我们的研究结果突出了 circRNA 在 NSCLC 中的重要诊断潜力。
