Muldoon Patrick F, Collet Guillaume, Eliseeva Svetlana V, Luo Tian-Yi, Petoud Stéphane, Rosi Nathaniel L
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique, 45071 Orléans, France.
J Am Chem Soc. 2020 May 13;142(19):8776-8781. doi: 10.1021/jacs.0c01426. Epub 2020 May 4.
While metal-organic frameworks (MOFs) have been identified as promising materials for sensitizing near-infrared emitting lanthanide ions (Ln) for biological imaging, long-wavelength excitation of such materials requires large, highly delocalized organic linkers or guest-chromophores. Incorporation of such species generally coincides with fewer Ln emitters per unit volume. Herein, the excitation bands of ytterbium-based MOFs are extended to 800 nm via the postsynthetic coupling of acetylene units to form a high density of conjugated π-systems throughout MOF pores. The resulting long wavelength excitation/absorption bands are a synergistic property of the composite material as they are not observed in the individual organic components after disassociation of the MOFs, thus circumventing the need for large organic chromophores. We demonstrate that the long wavelength excitation and emission properties of these modified MOFs are maintained in the biological conditions of cell culture (aqueous environment, salts, heating), pointing toward their promising use for biological imaging applications.
虽然金属有机框架（MOFs）已被确认为用于生物成像中敏化近红外发光镧系离子（Ln）的有前景的材料，但此类材料的长波长激发需要大的、高度离域的有机连接体或客体发色团。掺入此类物质通常伴随着每单位体积中Ln发射体数量的减少。在此，通过乙炔单元的后合成偶联，将基于镱的MOFs的激发带扩展至800 nm，从而在整个MOF孔中形成高密度的共轭π体系。所得的长波长激发/吸收带是复合材料的一种协同性质，因为在MOFs解离后，在各个有机组分中未观察到这些带，从而避免了对大有机发色团的需求。我们证明，这些改性MOFs的长波长激发和发射性质在细胞培养的生物条件（水性环境、盐、加热）下得以保持，这表明它们在生物成像应用方面具有广阔的应用前景。
