Fang Weina, Xie Mo, Hou Xiaoling, Liu Xiaoguo, Zuo Xiaolei, Chao Jie, Wang Lianhui, Fan Chunhai, Liu Huajie, Wang Lihua
School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, and Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
Key Laboratory for Organic Electronics & Information Dis-plays (KLOEID), Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, Nanjing 210046, China.
J Am Chem Soc. 2020 May 13;142(19):8782-8789. doi: 10.1021/jacs.0c01254. Epub 2020 Apr 30.
Ultraviolet (UV) light has long been known to damage nucleic acids. In this work, a DNA origami radiometer has been developed for measuring UV exposure by monitoring the morphological evolution of DNA origami nanostructures. Unlike linear DNA strands that tend to be degraded into small segments upon UV exposure, the structural complexity and interstrand connectivity of DNA origami remarkably alter the pathway of UV-induced DNA damage. A general pathway of expansion, distortion, and final disintegration is observed for DNA origami regardless of their shape and size; however the deformation kinetics is positively correlated with the number of nicks in the nanostructure. This structural continuity-dependent deformation can be translated into a DNA-based radiometer for measuring UV dose in the environment.
长期以来，人们都知道紫外线（UV）会损伤核酸。在这项工作中，一种DNA折纸辐射计已被开发出来，用于通过监测DNA折纸纳米结构的形态演变来测量紫外线暴露量。与线性DNA链在紫外线照射下往往会降解成小片段不同，DNA折纸的结构复杂性和链间连接性显著改变了紫外线诱导的DNA损伤途径。无论DNA折纸的形状和大小如何，都观察到了一种普遍的扩展、变形和最终解体的途径；然而，变形动力学与纳米结构中的切口数量呈正相关。这种依赖于结构连续性的变形可以转化为一种基于DNA的辐射计，用于测量环境中的紫外线剂量。
