He Zhimei, Shi Kejun, Li Jinggang, Chao Jie
Key Laboratory for Organic Electronics & Information Displays (KLOEID), Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, Nanjing 210023, China.
Smart Health Big Data Analysis and Location Services Engineering Research Center of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts & Telecommunications, Nanjing 210023, China.
iScience. 2023 Apr 10;26(5):106638. doi: 10.1016/j.isci.2023.106638. eCollection 2023 May 19.
Since the pioneering work of immobile DNA Holliday junction by Ned Seeman in the early 1980s, the past few decades have witnessed the development of DNA nanotechnology. In particular, DNA origami has pushed the field of DNA nanotechnology to a new level. It obeys the strict Watson-Crick base pairing principle to create intricate structures with nanoscale accuracy, which greatly enriches the complexity, dimension, and functionality of DNA nanostructures. Benefiting from its high programmability and addressability, DNA origami has emerged as versatile nanomachines for transportation, sensing, and computing. This review will briefly summarize the recent progress of DNA origami, two-dimensional pattern, and three-dimensional assembly based on DNA origami, followed by introduction of its application in nanofabrication, biosensing, drug delivery, and computational storage. The prospects and challenges of assembly and application of DNA origami are also discussed.
自20世纪80年代初内德·西曼开创固定DNA霍利迪连接体的工作以来，过去几十年见证了DNA纳米技术的发展。特别是，DNA折纸术将DNA纳米技术领域提升到了一个新水平。它遵循严格的沃森-克里克碱基配对原则，以纳米级精度创建复杂结构，极大地丰富了DNA纳米结构的复杂性、维度和功能性。得益于其高度的可编程性和可寻址性，DNA折纸术已成为用于运输、传感和计算的多功能纳米机器。本文将简要总结DNA折纸术、基于DNA折纸术的二维图案和三维组装的最新进展，随后介绍其在纳米制造、生物传感、药物递送和计算存储中的应用。还讨论了DNA折纸术组装和应用的前景与挑战。
