Physik Department and Institute for Advanced Study, Technische Universität München, Am Coulombwall 4a, 85748, Garching near Munich, Germany.
Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Angew Chem Int Ed Engl. 2017 Nov 27;56(48):15324-15328. doi: 10.1002/anie.201708722. Epub 2017 Oct 30.
We demonstrate direct observation of the dynamic opening and closing behavior of photocontrollable DNA origami nanoscissors using high-speed atomic force microscopy (HS-AFM). First the conformational change between the open and closed state controlled by adjustment of surrounding salt concentration could be directly observed during AFM scanning. Then light-responsive moieties were incorporated into the nanoscissors to control these structural changes by photoirradiation. Using photoswitchable DNA strands, we created a photoresponsive nanoscissors variant and were able to distinguish between the open and closed conformations after respective irradiation with ultraviolet (UV) and visible (Vis) light by gel electrophoresis and AFM imaging. Additionally, these reversible changes in shape during photoirradiation were directly visualized using HS-AFM. Moreover, four photoswitchable nanoscissors were assembled into a scissor-actuator-like higher-order object, the configuration of which could be controlled by the open and closed switching induced by irradiation with UV and Vis light.
我们使用高速原子力显微镜（HS-AFM）直接观察到光控 DNA 折纸纳米剪刀的动态开合行为。首先，在 AFM 扫描过程中，可以直接观察到周围盐浓度调节控制的开/合状态之间的构象变化。然后，将光响应部分掺入纳米剪刀中，通过光照射来控制这些结构变化。我们使用光致变色 DNA 链创建了一个光响应纳米剪刀变体，并通过凝胶电泳和 AFM 成像，在分别用紫外线 (UV) 和可见光 (Vis) 照射后，能够区分开/合构象。此外，使用 HS-AFM 可以直接可视化光照射过程中的形状可逆变化。此外，四个光致变色纳米剪刀组装成类似剪刀执行器的高阶物体，其构型可以通过 UV 和 Vis 光照射诱导的开/合切换来控制。
