School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
Department of Chemistry and Chemical Engineering, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.
J Am Chem Soc. 2017 Feb 1;139(4):1380-1383. doi: 10.1021/jacs.6b10821. Epub 2017 Jan 20.
The responses of DNA origami conformation to UV radiation of different wavelengths and doses are investigated. Short- and medium-wavelength UV light can cause photo-lesions in DNA origami. At moderate doses, the lesions do not cause any visible defects in the origami, nor do they significantly affect the hybridization capability. Instead, they help relieve the internal stress in the origami structure and restore it to the designed conformation. At high doses, staple dissociation increases which causes structural disintegration. Long-wavelength UV does not show any effect on origami conformation by itself. We show that this UV range can be used in conjunction with photoactive molecules for photo-reconfiguration, while avoiding any damage to the DNA structures.
研究了 DNA 折纸构象对不同波长和剂量的紫外线辐射的响应。短波长和中波长的紫外线会在 DNA 折纸中造成光损伤。在中等剂量下，损伤不会在折纸中造成任何可见的缺陷，也不会显著影响杂交能力。相反，它们有助于缓解折纸结构的内部应力，并使其恢复到设计的构象。在高剂量下，订书钉的解离增加，导致结构解体。长波长的紫外线本身不会对折纸构象产生任何影响。我们表明，这个紫外线范围可以与光活性分子一起用于光重构，同时避免对 DNA 结构造成任何损害。
