Reconstructive and Plastic Surgery, General Hospital of Northern Theater Command, Shenyang, P.R.China.
Aging (Albany NY). 2020 Apr 20;12(8):7056-7065. doi: 10.18632/aging.103063.
The carbon dioxide (CO) lattice laser has been successfully used to treat facial skin photoaging induced by UV light. In this study, we analyzed the effect of CO lattice laser irradiation on skin photoaging, and investigated the underlying mechanisms. Our results demonstrate that the laser promoted collagen synthesis and proliferation of primary human skin fibroblasts, inhibited cell senescence, and induced expression of superoxide dismutase (SOD) and the signaling protein SMAD3. In addition, this laser reversed cell cycle arrest and fibroblast apoptosis induced by UVB irradiation, and restored fibroblast proliferation inhibited by SMAD3 silencing. Using a rat model of photoaging, our results show that the laser increased collagen expression and dermal thickness, demonstrating that the CO lattice laser has a profound therapeutic effect on photoaged skin. Together, our and data show that the CO lattice laser can reverse the skin aging caused by UVB, and indicate that this effect is mediated through SMAD3.
二氧化碳（CO）点阵激光已成功用于治疗紫外线引起的面部皮肤光老化。在这项研究中，我们分析了 CO 点阵激光照射对皮肤光老化的影响，并探讨了其潜在机制。我们的结果表明，该激光促进了原代人皮肤成纤维细胞的胶原合成和增殖，抑制了细胞衰老，并诱导了超氧化物歧化酶（SOD）和信号蛋白 SMAD3 的表达。此外，该激光逆转了 UVB 照射诱导的细胞周期停滞和成纤维细胞凋亡，并恢复了 SMAD3 沉默抑制的成纤维细胞增殖。在光老化大鼠模型中，我们的结果表明，激光增加了胶原蛋白的表达和真皮厚度，表明 CO 点阵激光对光老化皮肤具有显著的治疗效果。综上所述，我们的数据表明 CO 点阵激光可以逆转 UVB 引起的皮肤衰老，并且表明这种作用是通过 SMAD3 介导的。
