College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Republic of Korea.
Biosolution Co., Seoul, 01811, Republic of Korea.
Arch Dermatol Res. 2017 Nov;309(9):697-707. doi: 10.1007/s00403-017-1768-6. Epub 2017 Aug 29.
Peptoids are a class of peptidomimetics whose pharmacological activities are widely investigated owing to their remarkable biological stability. However, the utilities of peptoids as cosmetic functional ingredients have not been fully explored. Here, we investigated anti-aging effects of PAL-12, a new hexa-peptoid, on UVB-induced photoaging in human dermal fibroblasts (HDFs) and a 3D reconstituted human full skin model, Keraskin-FT™. PAL-12 suppressed matrix metalloproteinase-1 (MMP-1) expression induced by UVB irradiation along with the attenuation of MMP-1 secretion as determined by ELISA assay. Interestingly PAL-12 slightly enhanced the expression levels of collagen-1 and fibronectin-1 in HDFs or Keraskin-FT™. In addition, PAL-12 prevented the decrease of cell viability following UVB irradiation. However, PAL-12 failed to affect ROS generation, cell necrosis and apoptosis significantly. Instead, PAL-12 suppressed UVB-induced activation of epidermal growth factor receptors (EGFR), extracellular signal-regulated kinase (ERK) and c-Jun, which may resulted in the attenuation of AP-1-promoted MMP-1 expression. Collectively, these results suggest that PAL-12 might be a novel cosmetic ingredient effective against UVB-induced skin photoaging.
肽缩氨酸是一类具有广泛研究价值的拟肽化合物，由于其具有显著的生物学稳定性，其药理学活性备受关注。然而，肽缩氨酸作为化妆品功能性成分的应用尚未得到充分探索。在这里，我们研究了一种新型六肽缩氨酸 PAL-12 对 UVB 诱导的人真皮成纤维细胞 (HDFs) 和 3D 重建的人全层皮肤模型 Keraskin-FT™ 光老化的抗衰老作用。PAL-12 抑制了 MMP-1 的表达，ELISA 检测结果显示 MMP-1 的分泌也随之减少。有趣的是，PAL-12 略微增加了 HDFs 或 Keraskin-FT™ 中胶原蛋白-1 和纤维连接蛋白-1 的表达水平。此外，PAL-12 防止了 UVB 照射后细胞活力的下降。然而，PAL-12 对 ROS 的产生、细胞坏死和凋亡没有显著影响。相反，PAL-12 抑制了 UVB 诱导的表皮生长因子受体 (EGFR)、细胞外信号调节激酶 (ERK) 和 c-Jun 的激活，这可能导致 AP-1 促进的 MMP-1 表达减少。综上所述，这些结果表明 PAL-12 可能是一种有效的新型化妆品成分，可用于对抗 UVB 诱导的皮肤光老化。
