Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Food Funct. 2019 Sep 1;10(9):6121-6134. doi: 10.1039/c9fo00610a. Epub 2019 Sep 9.
Collagen hydrolysate has been widely used as a nutraceutical agent against skin aging and has gained increasing attention. Previous research has suggested that oral administration of antioxidant collagen peptides (ACPs) exerted beneficial effects on the photoaging skin structure and collagen. However, the bioactive components in ACP metabolites that are responsible for the repair effects have not been elucidated. In this study, serum containing collagen peptides (CPS) after oral administration and collagen peptides isolated from serum metabolites (SCP) were collected and their effects on cell proliferation, hyaluronic acid secretion and the collagen synthesis pathway in UVA-induced skin fibroblasts (ESF) were evaluated. Furthermore, hydroxyproline (Hyp)-containing collagen peptides from SCP were analyzed and their repair effects were examined. The repair effects of ACP metabolites in serum differed depending on the preparation method and SCP were the active components responsible for the repair effects. SCP displayed repair effects by activating the TGF-β/Smad pathway to promote procollagen synthesis and suppressing AP-1, MMP-1 and MMP-3 protein expression to prevent collagen degradation, in which SHCP exhibited the strongest bioactivity. In addition, SCP showed repair effects by reactive oxygen species (ROS) scavenging activity and preserving the endogenous antioxidant defense systems. Furthermore, IO (Ile-Hyp) and AOG (Ala-Hyp-Gly) were identified as the active peptides promoting procollagen synthesis by activating the TGF-β/Smad3 pathway. These results may be useful in screening of anti-photoaging factors in metabolites and producing highly efficient collagen peptide products.
胶原蛋白水解物已被广泛用作对抗皮肤衰老的营养保健品，并受到越来越多的关注。先前的研究表明，口服抗氧化胶原蛋白肽（ACPs）对光老化皮肤结构和胶原蛋白具有有益作用。然而，负责修复作用的 ACP 代谢物中的生物活性成分尚未阐明。在这项研究中，收集了口服含胶原蛋白肽的血清（CPS）和从血清代谢物中分离的胶原蛋白肽（SCP），并评估了它们对 UVA 诱导的皮肤成纤维细胞（ESF）中细胞增殖、透明质酸分泌和胶原蛋白合成途径的影响。此外，还分析了 SCP 中含有羟脯氨酸（Hyp）的胶原蛋白肽，并检查了它们的修复作用。血清中 ACP 代谢物的修复作用取决于制备方法，SCP 是负责修复作用的活性成分。SCP 通过激活 TGF-β/Smad 途径促进前胶原蛋白合成并抑制 AP-1、MMP-1 和 MMP-3 蛋白表达来防止胶原蛋白降解，其中 SHCP 表现出最强的生物活性。此外，SCP 通过清除活性氧（ROS）和维持内源性抗氧化防御系统来显示修复作用。此外，鉴定出 IO（Ile-Hyp）和 AOG（Ala-Hyp-Gly）是通过激活 TGF-β/Smad3 途径促进前胶原蛋白合成的活性肽。这些结果可能有助于筛选代谢物中的抗光老化因子，并生产高效的胶原蛋白肽产品。
