文献检索，用中文搜 PubMed

Metabolomics has been applied to diagnose diseases, predict disease progression, and design therapeutic strategies in various areas of medicine. However, it remains to be applied to the ocular surface diseases, where biological samples are often of limited quantities. We successfully performed proof-of-concept metabolomics assessment of volume-limited cytology samples from a clinical form of chronic inflammatory cicatrizing conjunctivitis, i.e., ocular MMP and discovered metabolic changes of signaling lipid mediators upon disease onset and progression. The metabolomics assessment revealed active oxylipins, lysophospholipids, fatty acids, and endocannabinoids alterations, from which potential biomarkers linked to inflammatory processes were identified. Possible underlying mechanisms such as dysregulated enzyme activities (e.g., lipoxygenases, cytochrome P450, and phospholipases) were suggested which may be considered as potential therapeutic targets in future studies. KEY MESSAGES: Metabolic profile of the ocular surface can be measured using impression cytology samples. Metabolomics analysis of ocular pemphigoid is presented for the first time. The metabolomics assessment of OCP patients revealed active oxylipins, lysophospholipids, fatty acids, and endocannabinoids alterations. Several oxylipins are identified as diagnostic biomarkers for OCP.

代谢组学已被应用于诊断疾病、预测疾病进展和设计各种医学领域的治疗策略。然而，它仍然有待应用于眼表疾病，其中生物样本通常数量有限。我们成功地对一种慢性炎症性瘢痕性结膜炎的临床形式，即眼表 MMP 的有限容量细胞学样本进行了概念验证代谢组学评估，并发现了疾病发生和进展时信号脂质介质的代谢变化。代谢组学评估显示活性氧化脂质、溶血磷脂、脂肪酸和内源性大麻素的改变，从中鉴定出与炎症过程相关的潜在生物标志物。提示可能存在酶活性失调等潜在机制（如脂氧合酶、细胞色素 P450 和磷脂酶），这些机制可能在未来的研究中被视为潜在的治疗靶点。