Laboratory for Fish and Mollusc Diseases, Hrvatski Veterinarski Institut, Zagreb, Croatia.
Virology Department Zagreb, Hrvatski Veterinarski Institut, Zagreb, Croatia.
J Fish Dis. 2020 Jun;43(6):673-685. doi: 10.1111/jfd.13163. Epub 2020 Apr 21.
Common carp (Cyprinus carpio) is a very important fish species for warm-water aquaculture in Croatia. All Croatian carp farms are subjected to a surveillance programme for the presence of koi herpesvirus (KHV), causing a deadly disease called koi herpesvirus disease (KHVD). However, there is no surveillance for other viral pathogens of importance like carp edema virus (CEV), a causative agent of koi sleepy disease (KSD). During regular testing within the KHVD surveillance programme, we tested samples for CEV simultaneously. The screening indicated possible outbreaks of KHVD and KSD. During 2016, KHVD broke out in an isolated area and soon thereafter a KHV eradication programme was successfully performed. However, during 2018 and 2019, two additional mortality events occurred in lakes in the southern part of Croatia during the spring. Samples from both events tested positive for CEV. An epidemiological investigation confirmed the introduction of infected carps from an infected farm to one of the lakes. To prevent the spreading of CEV into open waters, it is of utmost importance to introduce CEV testing before fish movement or to perform regular testing of all carp farms in the country to determine CEV prevalence for the purpose of implementation of control measures.
鲤鱼（Cyprinus carpio）是克罗地亚温水水产养殖中非常重要的鱼类。所有克罗地亚的鲤鱼养殖场都受到锦鲤疱疹病毒（KHV）存在的监测计划的监测，这种病毒会导致一种名为锦鲤疱疹病毒病（KHVD）的致命疾病。然而，对于其他重要的病毒性病原体，如鲤鱼水肿病毒（CEV），并没有进行监测，CEV 是锦鲤昏睡病（KSD）的病原体。在 KHVD 监测计划的常规检测中，我们同时对 CEV 样本进行了检测。筛查结果表明可能爆发了 KHVD 和 KSD。2016 年，KHVD 在一个孤立的地区爆发，随后成功实施了 KHV 根除计划。然而，在 2018 年和 2019 年，克罗地亚南部的两个湖泊在春季又发生了两起额外的死亡事件。来自这两个事件的样本检测出 CEV 呈阳性。流行病学调查证实，受感染的鲤鱼是从一个受感染的养殖场传入其中一个湖泊的。为了防止 CEV 传播到开放水域，在鱼类移动之前进行 CEV 检测，或对该国所有鲤鱼养殖场进行定期检测，以确定 CEV 的流行率，以便实施控制措施，这一点至关重要。
