State Key Laboratory of Microbial Resources, Institute of Microbiology, CAS, Beijing, 100101, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Curr Microbiol. 2020 Aug;77(8):1939-1944. doi: 10.1007/s00284-020-01983-8. Epub 2020 Apr 21.
A Gram-negative, aerobic, motile, non-spore-forming and rod-shaped bacterium, designated strain B18-69, was isolated from oil-well production liquid in Baolige oilfield, China. The strain was able to grow at pH 6-9.5 (optimum at pH 7), in 0-4% (w/v) NaCl (optimum at 0.5-1%, w/v) and at 35-60 °C (optimum at 55 °C). Major cellular fatty acids were C, C cyclo ω8c, C cyclo and C ω7c. The predominant respiratory quinone was ubiquinone 8. Major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylcholine (PC). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B18-69 was most closely related to Tepidiphilus margaritifer DSM 15129 (98.8% similarity). The draft genome of strain B18-69 was composed of 2,250,419 bp, and the G+C content was 64.6 mol%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain B18-69 and T. margaritifer DSM 15129 were 90.9% and 68.9%, respectively. Genotypic and phenotypic features indicate that strain B18-69 represents a novel species of the genus Tepidiphilus, for which the name Tepidiphilus baoligensis sp. nov. is proposed. The type strain is B18-69 (= CGMCC 1.13573 = KCTC 62782).
一株革兰氏阴性、需氧、能动、不产芽孢的杆状细菌，被命名为 B18-69 菌株，从中国宝利格油田的油井生产液中分离得到。该菌株能在 pH 值为 6-9.5 之间生长（最佳 pH 值为 7），在 0-4%（w/v）NaCl（最佳浓度为 0.5-1%，w/v）和 35-60°C（最佳温度为 55°C）下生长。主要细胞脂肪酸为 C、C 环 ω8c、C 环和 C ω7c。主要呼吸醌为泛醌 8。主要极性脂类为磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、双磷脂酰甘油（DPG）和磷脂酰胆碱（PC）。基于 16S rRNA 基因序列的系统发育分析表明，B18-69 菌株与 Tepidiphilus margaritifer DSM 15129 最为密切（相似度为 98.8%）。B18-69 菌株的基因组草图由 2250419 个碱基对组成，G+C 含量为 64.6 mol%。B18-69 菌株与 T. margaritifer DSM 15129 之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 90.9%和 68.9%。基因型和表型特征表明，B18-69 菌株代表了 Tepidiphilus 属的一个新种，命名为 Tepidiphilus baoligensis sp. nov.。其模式菌株为 B18-69（= CGMCC 1.13573 = KCTC 62782）。
