China General Microbiological Culture Collection Center, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
College of Engineering, Peking University, Beijing 100871, PR China.
Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005064.
A pink, ovoid-shaped, Gram-stain-negative, strictly aerobic and motile bacterial strain, designated ROY-5-3, was isolated from an oil production mixture from Yumen Oilfield in PR China. The strain grew at 4-42 °C (optimum, 30 °C), at pH 5-10 (optimum, 7) and with 0-5 % (w/v) NaCl (optimum, 0%). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that ROY-5-3 belongs to the genus and shared the highest pairwise similarities with CW67 (98.1%), BU-1 (97.8%), K-20 (97.7%) and HS-69 (97.3%). The average nucleotide identity and digital DNA-DNA hybridization values between ROY-5-3 and other related type strains of species were less than 84.08 and 28.60 %, respectively, both below the species delineation threshold. Pan-genomic analysis showed that the novel isolate ROY-5-3 shared 3265 core gene families with the four closely related type strains in , and the number of strain-specific gene families was 513. The major fatty acids were identified as summed feature 8 (C 6/C 7), summed feature 3 (C 6/C 7) and C. Strain ROY-5-3 contained Q-10 as the main ubiquinone and the genomic DNA G+C content was 69.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Based on the phylogenetic, morphological, physiological, chemotaxonomic and genome analyses, strain ROY-5-3 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is ROY-5-3 (=CGMCC 1.13459 =KCTC 82484).
一株粉色、卵形、革兰氏阴性、严格需氧和能动的细菌菌株，被命名为 ROY-5-3，从中国玉门油田的采油混合物中分离得到。该菌株在 4-42°C（最佳温度为 30°C）、pH 值 5-10（最佳值为 7）和 0-5%（w/v）NaCl（最佳值为 0%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析结果表明，ROY-5-3 属于 属，与 CW67（98.1%）、BU-1（97.8%）、K-20（97.7%）和 HS-69（97.3%）最为相似。ROY-5-3 与其他相关 种的模式菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值均小于 84.08%和 28.60%，均低于种的界定阈值。泛基因组分析表明，新型分离株 ROY-5-3 与 4 株密切相关的模式菌株共享 3265 个核心基因家族，而菌株特有的基因家族数量为 513 个。主要脂肪酸为 C6/C7 总和特征 8、C6/C7 总和特征 3 和 C。ROY-5-3 菌株含有 Q-10 作为主要的泛醌，基因组 DNA G+C 含量为 69.8 mol%。主要极性脂类为二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油。基于系统发育、形态学、生理学、化学分类学和基因组分析，菌株 ROY-5-3 代表了 属的一个新种，建议将其命名为 sp. nov.。该模式菌株为 ROY-5-3（=CGMCC 1.13459=KCTC 82484）。
