Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Immunopharmacol Immunotoxicol. 2020 Jun;42(3):272-279. doi: 10.1080/08923973.2020.1755308. Epub 2020 Apr 23.
Ischemia/reperfusion can cause injury to tissues and compromise functionality of organs due to inflammatory processes. Significantly, development of these effects in kidney tissue has been a challenging issue that leads to acute renal injury. In this study, anti-inflammatory, anti-oxidative, and protective features of dapsone on kidney ischemia/reperfusion injury were investigated. Renal ischemia was induced in rats by bilateral renal arteries clamping for 45 min followed by 24 h reperfusion phase. The effects of different doses of dapsone (1, 3, 10 mg/kg) on ischemia/reperfusion injury in kidney tissue were investigated by targeting BUN, Creatinine, LDH, MDA, MPO, IL-1β, TNF-α, and NFκB. In addition histopathological examination was performed by H&E staining method. Comparing the findings of this study showed significant reduction in BUN and LDH in 10 mg/kg dapsone received groups, and Cr, MDA, and MPO in 3 mg/kg dapsone received groups. The serum level of TNF-α was significantly decreased with both doses of 3 and 10 mg/kg dapsone. The same results were observed in the serum level of IL-1β and NFκB. Besides, remarkable improvement in histological damages was also observed with dapsone treatment. These results support the hypothesis that the positive effects of dapsone on the renal ischemia/reperfusion injury are mediated by modulating inflammatory cascades.
缺血/再灌注会导致组织损伤，并因炎症过程而损害器官功能。值得注意的是，肾脏组织中这些效应的发展一直是一个具有挑战性的问题，会导致急性肾损伤。在这项研究中，研究了氨苯砜对肾缺血/再灌注损伤的抗炎、抗氧化和保护作用。通过双侧肾动脉夹闭 45 分钟，然后再灌注 24 小时来诱导大鼠肾脏缺血。通过靶向 BUN、肌酐、LDH、MDA、MPO、IL-1β、TNF-α 和 NFκB，研究了不同剂量的氨苯砜（1、3、10mg/kg）对肾脏组织缺血/再灌注损伤的影响。此外，还通过 H&E 染色法进行了组织病理学检查。比较研究结果表明，10mg/kg 氨苯砜组 BUN 和 LDH 显著降低，3mg/kg 氨苯砜组 Cr、MDA 和 MPO 显著降低。两种剂量的 3 和 10mg/kg 氨苯砜均可显著降低 TNF-α 血清水平。IL-1β 和 NFκB 的血清水平也观察到相同的结果。此外，氨苯砜治疗还显著改善了组织损伤。这些结果支持这样一种假设，即氨苯砜对肾缺血/再灌注损伤的积极作用是通过调节炎症级联反应来介导的。
