Miller J E, Barbour S D
J Bacteriol. 1977 Apr;130(1):160-6. doi: 10.1128/jb.130.1.160-166.1977.
The abilities of rec+, recB- recC-, recA-, and recA- recB- rec C- strains to support growth of bacteriophage T4, to take up oxygen, and to maintain cell integrity have been measured. (i) With respect to bacteriophage T4 growth, T4 phage is produced with identical lysis time in single -step growth curves with all strains tested. rec- strains show reduced phage production (fewer infected centers), but the average burst size per infected center is the same for all strains tested. Some rec- cells are unable to produce any phage, whereas the remainder of the rec-cells produce phage as rapidly and as efficiently as rec+ cells. (ii) With respect to oxygen consumption, rec- strains are deficient relative to the rec+ control to the same extent as the deficiency in phage production by theculture. The reduction in oxygen consumption is coordinate with reduction in rate of mass increase of the rec- culture. (iii) With respect to cell integrity, rec- cultures show increased lysis as measured by release of beta-galactosidase into the medium. The kinetics of release suggest that rec- nondividing cells all eventually lyse. These results are most consistent with the idea that rec- residually dividing cells and viable cells are metabolically normal according to the parameters we have measured, whereas nondividing cells are metabolically inactive.
已测定rec⁺、recB⁻ recC⁻、recA⁻以及recA⁻ recB⁻ recC⁻菌株支持噬菌体T4生长、摄取氧气和维持细胞完整性的能力。(i) 关于噬菌体T4生长，在所有测试菌株的一步生长曲线中，T4噬菌体产生时的裂解时间相同。rec⁻菌株显示噬菌体产量降低（感染中心较少），但每个感染中心的平均爆发量对于所有测试菌株而言是相同的。一些rec⁻细胞无法产生任何噬菌体，而其余rec⁻细胞产生噬菌体的速度和效率与rec⁺细胞相同。(ii) 关于氧气消耗，rec⁻菌株相对于rec⁺对照的缺陷程度与培养物中噬菌体产量的缺陷程度相同。氧气消耗的减少与rec⁻培养物质量增加速率的降低是协同的。(iii) 关于细胞完整性，通过β-半乳糖苷酶释放到培养基中的量来衡量，rec⁻培养物显示出增加的裂解。释放动力学表明rec⁻非分裂细胞最终都会裂解。这些结果与以下观点最为一致：根据我们测量的参数，rec⁻残余分裂细胞和活细胞在代谢上是正常的，而非分裂细胞在代谢上是无活性的。
