大肠杆菌K-12 hisT突变体的生化与调控特性
Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.
作者信息
Bruni C B, Colantuoni V, Sbordone L, Cortese R, Blasi F
出版信息
J Bacteriol. 1977 Apr;130(1):4-10. doi: 10.1128/jb.130.1.4-10.1977.
Abstract
Escherichia coli K-12 hisT mutants were isolated, and their properties were studied. These mutants are derepressed for the histidine operon, map close to the purF locus at about 49.5 min on the E. coli linkage map, and lack pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several transfer ribonucleic acid species, as evidenced by the altered elution profile on reversed-phase chromatography and resistance to amino acid analogues. Finally, the hisT mutants studied have a reduced growth rate that appears to be linked to hisT, although it is not known whether it is due to the same mutation. The normal generation time can be restored by supplementing the medium with adenine, uracil, and isoleucine.
摘要
分离出了大肠杆菌K-12 hisT突变体，并对其特性进行了研究。这些突变体的组氨酸操纵子去阻遏，在大肠杆菌连锁图谱上位于约49.5分钟处，靠近purF位点，并且缺乏假尿苷酸合成酶活性。这种酶的缺陷导致几种转移核糖核酸种类的反密码子环中没有假尿苷，这在反相色谱上洗脱图谱的改变以及对氨基酸类似物的抗性中得到了证明。最后，所研究的hisT突变体生长速率降低，这似乎与hisT有关，尽管尚不清楚这是否是由同一突变引起的。通过在培养基中添加腺嘌呤、尿嘧啶和异亮氨酸，可以恢复正常的代时。
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