Silva Natacha, Ivamoto-Suzuki Suzana Tiemi, Camargo Paula Oliveira, Rosa Raíssa Scalzoni, Pereira Luiz Filipe Protasio, Domingues Douglas Silva
Departamento de Biodiversidade, Instituto de Biociências, Universidade Estadual Paulista, UNESP, 13506-900 Rio Claro-SP, Brazil.
Laboratório de Biotecnologia Vegetal, Empresa Brasileira de Pesquisa Agropecuária (Embrapa-Café), 86047-902 Londrina-PR, Brazil.
Plants (Basel). 2020 Apr 19;9(4):525. doi: 10.3390/plants9040525.
Terpenoids are a diverse class of metabolites that impact plant metabolism in response to environmental cues. They are synthesized either via a predominantly cytosolic (MVA) pathway or a plastidic pathway (MEP). In , several enzymes from the MVA and MEP pathways are encoded by gene families, excluding and , which are single-copy genes. In this study, we assess the diversity, evolution and expression of and genes in selected angiosperms and in particular. Evolutionary analysis revealed that and underwent purifying selection, but the selection effect for was stronger than it was fo. Digital gene expression (DGE) profile analysis of six species revealed that expression levels of in flowers and roots were high, whereas for peak values were observed in leaves. In , both genes were highly expressed in flowers, and was upregulated in response to methyl jasmonate. DGE data were validated by assessing gene expression in selected organs, and by plants treated with hexanoic acid (Hx) using RT-qPCR. expression was upregulated in roots treated with Hx. was downregulated in leaves by Hx treatment in a genotype-specific manner, indicating a differential response to priming.
萜类化合物是一类多样的代谢产物，它们响应环境信号影响植物代谢。它们通过主要位于胞质的（甲羟戊酸，MVA）途径或质体途径（2-C-甲基-D-赤藓糖醇-4-磷酸，MEP）合成。在[具体物种]中，MVA和MEP途径的几种酶由基因家族编码，但除外[两个特定基因]，它们是单拷贝基因。在本研究中，我们评估了[特定基因]在选定被子植物中的多样性、进化和表达，特别是[某一特定植物]。进化分析表明，[相关基因]经历了纯化选择，但对[一个基因]的选择效应强于对[另一个基因]的选择效应。对六个物种的数字基因表达（DGE）谱分析表明，[一个基因]在花和根中的表达水平较高，而[另一个基因]在叶中观察到峰值。在[某一特定植物]中，两个基因在花中均高表达，并且[一个基因]响应茉莉酸甲酯而上调。通过评估选定器官中的基因表达以及用己酸（Hx）处理的植物，使用逆转录定量PCR（RT-qPCR）验证了DGE数据。Hx处理的根中[一个基因]表达上调。Hx处理以基因型特异性方式使叶中的[另一个基因]下调，表明对引发的差异响应。
