Fernando Ilekuttige Priyan Shanura, Dias Mawalle Kankanamge Hasitha Madhawa, Madusanka Dissanayaka Mudiyanselage Dinesh, Han Eui Jeong, Kim Min Ju, Jeon You-Jin, Lee Kyounghoon, Cheong Sun Hee, Han Young Seok, Park Sang Rul, Ahn Ginnae
Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea.
Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea.
Antioxidants (Basel). 2020 Apr 21;9(4):340. doi: 10.3390/antiox9040340.
Ultraviolet B (UVB) radiation-induced oxidative skin cell damage is a major cause of photoaging. In the present study, a low molecular weight fucoidan fraction (SHC4) was obtained from by Celluclast-assisted extraction, followed by step gradient ethanol precipitation. The protective effect of SHC4 was investigated in human keratinocytes against UVB-induced oxidative stress. The purified fucoidan was characterized by Fourier-transform infrared spectroscopy (FTIR), H nuclear magnetic resonance (NMR), agarose gel-based molecular weight analysis and monosaccharide composition analysis. SHC4 had a mean molecular weight of 60 kDa, with 37.43% fucose and 28.01 ± 0.50% sulfate content. The structure was mainly composed of α-L-Fucp-(1→4) linked fucose units. SHC4 treatment dose-dependently reduced intracellular reactive oxygen species (ROS) levels and increased the cell viability of UVB exposed HaCaT keratinocytes. Moreover, SHC4 dose-dependently inhibited UVB-induced apoptotic body formation, sub-G accumulation of cells and DNA damage. Inhibition of apoptosis was mediated via the mitochondria-mediated pathway, re-establishing the loss of mitochondrial membrane potential. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defense via nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Further studies may promote the use of SHC4 as an active ingredient in cosmetics and nutricosmetics.
紫外线B（UVB）辐射诱导的皮肤细胞氧化损伤是光老化的主要原因。在本研究中，通过纤维素酶辅助提取，随后进行分步梯度乙醇沉淀，从[具体来源未给出]中获得了一种低分子量岩藻依聚糖级分（SHC4）。研究了SHC4对人角质形成细胞抵抗UVB诱导的氧化应激的保护作用。通过傅里叶变换红外光谱（FTIR）、氢核磁共振（NMR）、基于琼脂糖凝胶的分子量分析和单糖组成分析对纯化的岩藻依聚糖进行了表征。SHC4的平均分子量为60 kDa，岩藻糖含量为37.43%，硫酸盐含量为28.01±0.50%。其结构主要由α-L-岩藻糖基-（1→4）连接的岩藻糖单元组成。SHC4处理剂量依赖性地降低了细胞内活性氧（ROS）水平，并提高了UVB照射的HaCaT角质形成细胞的细胞活力。此外，SHC4剂量依赖性地抑制了UVB诱导的凋亡小体形成、细胞亚G期积累和DNA损伤。凋亡的抑制是通过线粒体介导的途径介导的，恢复了线粒体膜电位的丧失。SHC4的UVB保护作用是通过核因子红细胞2相关因子2（Nrf2）/血红素加氧酶-1（HO-1）信号通路增强细胞内抗氧化防御来实现的。进一步的研究可能会促进将SHC4用作化妆品和营养化妆品中的活性成分。
