Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Biol Macromol. 2020 Sep 15;159:773-781. doi: 10.1016/j.ijbiomac.2020.05.132. Epub 2020 May 20.
The escalation of fine particulate matter (PM) air pollution has recently become a global concern. Evidence is fast accumulating on PM exposure-related skin damage. The present study explored the therapeutic potentials of fucoidan purified from Sargassum natans against damaging effects of PM exposure on human HaCaT keratinocytes. Fucoidan (SNF7) was purified from S. natans by an enzyme-assisted extraction and purified by anion exchange chromatography. SNF7 (≈50 kDa) was identified as a fucoidan containing 70.97% fucose and 36.41 ± 0.59% of sulfate. Treatment of fine dust from Beijing, China (CFD) increased intracellular ROS levels in HaCaT cells triggering DNA damage and apoptosis. Treatment of SNF7 dose-dependently attenuated CFD-induced surge of intracellular ROS levels in keratinocytes by increasing antioxidant defense enzymes. Moreover, SNF7 chelated metal ions Pb, Ba, Sr, Cu, Fe, and Ca coming from CFD. The results substantiated the potential therapeutic effects of SNF7 against CFD-induced oxidative stress. Further studies could promote SNF7's use as an active ingredient in cosmetics.
细颗粒物（PM）空气污染的加剧最近成为了一个全球性的关注点。越来越多的证据表明，PM 暴露与皮肤损伤有关。本研究探讨了从马尾藻中提取的褐藻糖胶对 PM 暴露对人 HaCaT 角质细胞的损伤作用的治疗潜力。褐藻糖胶（SNF7）通过酶辅助提取从马尾藻中纯化，然后通过阴离子交换层析进一步纯化。SNF7（约 50 kDa）被鉴定为一种褐藻糖胶，含有 70.97%的岩藻糖和 36.41±0.59%的硫酸盐。来自中国北京的细粉尘（CFD）处理增加了 HaCaT 细胞内的 ROS 水平，引发了 DNA 损伤和细胞凋亡。SNF7 的处理剂量依赖性地降低了 CFD 诱导的角质细胞内 ROS 水平的增加，增加了抗氧化防御酶。此外，SNF7 螯合了来自 CFD 的金属离子 Pb、Ba、Sr、Cu、Fe 和 Ca。这些结果证实了 SNF7 对 CFD 诱导的氧化应激的潜在治疗作用。进一步的研究可以促进 SNF7 作为化妆品有效成分的使用。
