Dias Mawalle Kankanamge Hasitha Madhawa, Madusanka Dissanayaka Mudiyanselage Dinesh, Han Eui Jeong, Kim Min Ju, Jeon You-Jin, Kim Hyun-Soo, Fernando Ilekuttige Priyan Shanura, Ahn Ginnae
Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea.
Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea.
Antioxidants (Basel). 2020 Jun 2;9(6):474. doi: 10.3390/antiox9060474.
The emergence of fine dust (FD) among air pollutants has taken a toll during the past few decades, and it has provided both controversy and a platform for open conversation amongst world powers for finding sustainable solutions and effective treatments for health issues. The present study emphasizes the protective effects of (-)-loliolide (HTT) isolated from against FD-induced oxidative stress in human HaCaT keratinocytes. The purification of (-)-loliolide was carried out by centrifugal partition chromatography. HTT did not show any cytotoxicity, and it further illustrated the potential to increase cell viability by reducing the reactive oxygen species (ROS) production in FD-stimulated keratinocytes. Furthermore, HTT suppressed FD-stimulated DNA damage and the formation of apoptotic bodies, and it reduced the population of cells in the sub-G apoptosis phase. FD-induced apoptosis was advancing through the mitochondria-mediated apoptosis pathway. The cytoprotective effects of the HTT against FD-stimulated oxidative damage is mediated through squaring the nuclear factor E2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) pathway, dose-dependently increasing HO-1 and NAD(P)H dehydrogenase (quinone) 1 (NQO1) levels in the cytosol while concomitantly improving the nuclear translocation of Nrf2. Future studies could implement the protective functionality of HTT in producing pharmaceuticals that utilize natural products and benefit the diseased.
在过去几十年中，细颗粒物（FD）作为空气污染物之一造成了严重危害，它引发了争议，也为世界各国寻找可持续解决方案和有效健康问题治疗方法提供了公开对话的平台。本研究强调了从[具体来源未给出]中分离出的（-）-洛利内酯（HTT）对人HaCaT角质形成细胞中FD诱导的氧化应激的保护作用。（-）-洛利内酯的纯化通过离心分配色谱法进行。HTT未显示出任何细胞毒性，并且进一步表明其具有通过减少FD刺激的角质形成细胞中活性氧（ROS）的产生来提高细胞活力的潜力。此外，HTT抑制了FD刺激的DNA损伤和凋亡小体的形成，并减少了亚G期凋亡细胞的数量。FD诱导的凋亡通过线粒体介导的凋亡途径进行。HTT对FD刺激的氧化损伤的细胞保护作用是通过激活核因子E2相关因子2（Nrf2）介导的血红素加氧酶-1（HO-1）途径来介导的，它剂量依赖性地增加了细胞溶质中HO-1和NAD（P）H脱氢酶（醌）1（NQO1）的水平，同时促进了Nrf2的核转位。未来的研究可以将HTT的保护功能应用于利用天然产物的药物生产中，造福患者。
