Laboratorio de Ecología Química, Instituto de Química, Pontificia Universidad Católica de Valparaíso, Avda. Universidad 330. Curauma, Valparaíso 2340000, Chile.
Facultad de Ciencias para el cuidado de la Salud, Universidad San Sebastián, General Lagos 1163, Valdivia 5090000, Chile.
Molecules. 2020 Jul 3;25(13):3054. doi: 10.3390/molecules25133054.
(Polygonaceae) (known as lady's thumb) is an annual morphologically variable weed that is widely distributed in Chile. The purpose of this study was to investigate the antifeedant potential of methanolic (MeOH), ethanolic (EtOH), and dichloromethane (DCM) extracts from the aerial parts of this plant collected in the Valparaíso and Curicó provinces (Chile) and relate this activity to the antioxidant capacity and the presence of phenolic compounds in the extracts. A phenolic profile based on HPLC-ESI-MS/MS allowed the identification of 26 phenolic compounds, most of them glycosyl derivatives of isorhamnetin, quercetin, and kaempferol. In addition, the total phenolic content (TP), total flavonoids (TF), and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion scavenging (O), ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) of the extracts are reported. The antifeedant potentials of the plant extracts were tested against , , , and insects for the first time. The activity against the aphid was significant for the DCM extracts of plants from Valparaíso and Curicó (settling % = 23% ± 4% and 23% ± 5%, respectively). The antifeedant activities against the beetle and the lepidoptera were significant for Valparaíso extracts, especially when tested against (IFP = 1.0 ± 0.0). Finally, the MeOH and EtOH extracts from Valparaíso plants reduced the diet consumption of the psilid ( < 0.05). The results showed that is a good source of flavonoids with some antioxidant capacities and has potential interest as botanical eco-friendly alternative with deterrent activity.
（Polygonaceae）（俗称拇指姑娘）是一种一年生的形态变异杂草，广泛分布于智利。本研究旨在研究智利瓦尔帕莱索和库里科省（智利）采集的该植物地上部分的甲醇（MeOH）、乙醇（EtOH）和二氯甲烷（DCM）提取物的抗食作用，并将这种活性与提取物的抗氧化能力和酚类化合物的存在联系起来。基于 HPLC-ESI-MS/MS 的酚类图谱允许鉴定出 26 种酚类化合物，其中大多数为异鼠李素、槲皮素和山奈酚的糖苷衍生物。此外，还报告了提取物的总酚含量（TP）、总类黄酮（TF）和抗氧化活性，这些活性通过 2,2-二苯基-1-苦基肼（DPPH）、超氧阴离子清除（O）、铁还原抗氧化能力（FRAP）和铜还原抗氧化能力（CUPRAC）来测量。首次报道了该植物提取物对 、 、 、 昆虫的抗食作用。DCM 提取物对来自瓦尔帕莱索和库里科的植物的蚜虫 具有显著的活性（沉降率分别为 23%±4%和 23%±5%）。对甲虫 和鳞翅目昆虫 的抗食活性对瓦尔帕莱索提取物具有显著意义，尤其是在测试对 时（IFP=1.0±0.0）。最后，来自瓦尔帕莱索植物的 MeOH 和 EtOH 提取物减少了粉虱 的饮食消耗（<0.05）。结果表明， 是一种具有一定抗氧化能力的黄酮类化合物的良好来源，具有作为具有驱避活性的植物环保替代物的潜在兴趣。
