Chen Rui-Rong, Liu Juan, Chen Zhe, Cai Wen-Jun, Li Xiu-Fen, Lu Chuan-Li
Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Academy of Sciences, Guangzhou 510316, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Evid Based Complement Alternat Med. 2020 Mar 30;2020:9861571. doi: 10.1155/2020/9861571. eCollection 2020.
In Southwestern China, the root of Roxb. has been employed as a folk medicine for treating various types of hepatitis and jaundice. The purpose of this study was to evaluate the hepatoprotective effects of anthraquinones extract from . root (AEMA) in carbon tetrachloride- (CCl-) induced liver injury in mice and identify the main bioactive components. Results indicated that AEMA pretreatment could significantly, in a dose-dependent manner, attenuate the increased levels of ALT and AST in mice serum induced by CCl. At doses of 100 and 200 mg/kg, AEMA exhibited significant suppression of the elevated hepatic levels of malondialdehyde (MDA), as well as marked upregulatory effects on the activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice exposed to CCl. However, AEMA treatment had no effect on the antioxidant enzyme catalase (CAT) or the nonenzymatic antioxidant glutathione (GSH). Furthermore, two anthraquinone constituents were isolated from AEMA and identified as soranjidiol and rubiadin-3-methyl ether. Soranjidiol exhibited similar protective effects to those of AEMA on liver damage induced by CCl. Overall, our research clearly demonstrated the hepatoprotective effects of the AEMA, and anthraquinones, particularly soranjidiol, should be considered as the main hepatoprotective principles of . . In addition, the underlying mechanism may be, at least in part, related to its antioxidant properties.
在中国西南部，某植物的根被用作治疗各种类型肝炎和黄疸的民间药物。本研究的目的是评估该植物根中蒽醌提取物（AEMA）对四氯化碳（CCl₄）诱导的小鼠肝损伤的保肝作用，并确定主要生物活性成分。结果表明，AEMA预处理能够以剂量依赖的方式显著减轻CCl₄诱导的小鼠血清中ALT和AST水平的升高。在100和200mg/kg剂量下，AEMA对CCl₄处理小鼠肝脏中丙二醛（MDA）水平的升高表现出显著抑制作用，同时对包括超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）在内的抗氧化酶活性有明显上调作用。然而，AEMA处理对抗氧化酶过氧化氢酶（CAT）或非酶抗氧化剂谷胱甘肽（GSH）没有影响。此外，从AEMA中分离出两种蒽醌成分，鉴定为索拉吉二醇和茜草素-3-甲醚。索拉吉二醇对CCl₄诱导的肝损伤表现出与AEMA相似的保护作用。总体而言，我们的研究清楚地证明了AEMA的保肝作用，蒽醌类化合物，特别是索拉吉二醇，应被视为该植物的主要保肝成分。此外，其潜在机制可能至少部分与其抗氧化特性有关。
