Pharmacology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalioubeya, 13736, Egypt.
Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Environ Sci Pollut Res Int. 2020 Jun;27(18):23026-23034. doi: 10.1007/s11356-020-08919-6. Epub 2020 Apr 23.
The present study aimed to investigate the possible mitigating effect of L-carnitine (LC) and/or α-tocopherol (Vit. E) administration against tilmicosin (TIL)-induced cardiotoxicity in rats. Fifty-six male albino rats were divided into seven groups according to LC, Vit. E, and/or TIL administration. Control, LC, and Vit. E groups were given saline, 150 mg LC/kg body weight (BW)/day and 100 mg Vit. E/kg BW/day, respectively, orally once daily for 15 days. The TIL group was administered saline orally once daily for 15 days and a single dose of TIL (75 mg/kg BW) subcutaneously (SC) on day 14 from the starting of the experimental period (15 days). The TIL-LC, TIL-Vit. E, and TIL-LC-Vit. E groups received 150 mg LC/kg BW/day, 100 mg Vit. E/kg BW/day, and 150 mg LC/kg BW pulse 100 mg Vit. E/kg BW, respectively, orally once daily for 15 days with TIL as described above. The results revealed that the administration of TIL significantly (P ≤ 0.05) raised serum activities of heart injury indicators, lactate dehydrogenase (LDH), creatine kinase (CK), and CK-MB with substantial increase (P ≤ 0.05) in the cardiac contents of malondialdehyde (MDA) and decreased in antioxidants. The pathological changes appeared in the form of necrotic muscle fibers and massive inflammatory cellular infiltrations in the cardiac muscle and increased the caspase-3 immunohistochemical expression in the heart tissues as well. These changes were ameliorated by LC and/or Vit. E administration. In conclusion, supplementation of LC and/or Vit. E ameliorated the cardiotoxicity of the TIL SC injection in the rat.
本研究旨在探讨左旋肉碱（LC）和/或α-生育酚（Vit. E）给药是否可能减轻替米考星（TIL）诱导的大鼠心脏毒性。56 只雄性白化大鼠根据 LC、Vit. E 和/或 TIL 给药情况分为 7 组。对照组、LC 组和 Vit. E 组分别给予生理盐水、150mg LC/kg 体重（BW）/天和 100mg Vit. E/kg BW/天，每天口服一次，共 15 天。TIL 组每天口服生理盐水 15 天，在实验开始后第 15 天（15 天）皮下（SC）单次给予 TIL（75mg/kg BW）。TIL-LC、TIL-Vit. E 和 TIL-LC-Vit. E 组分别给予 150mg LC/kg BW/天、100mg Vit. E/kg BW/天和 150mg LC/kg BW 脉冲 100mg Vit. E/kg BW/天，每天口服一次，如上所述给予 TIL。结果表明，TIL 给药显著（P≤0.05）升高血清心脏损伤标志物肌酸激酶（CK）、乳酸脱氢酶（LDH）和 CK-MB 的活性，心脏丙二醛（MDA）含量显著升高（P≤0.05），抗氧化剂减少。病理变化表现为心肌坏死肌纤维和大量炎症细胞浸润，并增加心脏组织中 caspase-3 的免疫组化表达。LC 和/或 Vit. E 给药可改善这些变化。结论：LC 和/或 Vit. E 的补充可改善 TIL SC 注射引起的大鼠心脏毒性。
