Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Department of Prevention and Control for Occupational Disease, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Int J Environ Res Public Health. 2020 Apr 22;17(8):2899. doi: 10.3390/ijerph17082899.
: The purpose of this paper was to clarify the association between genetic variation in the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene and the risk of noise-induced hearing loss (NIHL). : A case-control study (633 cases and 625 controls) was conducted in this study. Logistic regression was used to analyze the relationships between environmental and individual factors and NIHL. Gene expression levels were compared among each rs6489721 genotype and between the case and control groups based on real-time fluorescence quantitative Polymerase Chain Reaction (PCR). : The T allele of rs6489721 was significantly associated with NIHL (odds ratio (OR) = 1.262, 95% confidence interval (CI) (1.066, 1.493), = 0.006) and showed strong associations in the codominant and dominant models (TT vs. CC: OR = 1.586, 95% CI (1.131, 2.225), = 0.008; TT vs. TC/CC: OR = 1.391, 95% CI (1.073, 1.804), = 0.013). The expression level of the TT genotype was significantly higher than that of the CC genotype ( = 0.012), and the expression of the case group was also higher than that of the control group ( = 0.013). : The homozygous risk allele (TT) of rs6489721 was associated with an enhanced expression, resulting in the development of NIHL in a Chinese population.
本研究旨在阐明甘油醛 3-磷酸脱氢酶（GAPDH）基因遗传变异与噪声性听力损失（NIHL）风险之间的关联。
该研究采用病例对照研究（633 例病例和 625 例对照）。采用 logistic 回归分析环境和个体因素与 NIHL 的关系。根据实时荧光定量聚合酶链反应（PCR），比较每个 rs6489721 基因型和病例组与对照组之间的基因表达水平。
rs6489721 的 T 等位基因与 NIHL 显著相关（比值比（OR）=1.262，95%置信区间（CI）（1.066，1.493），=0.006），在共显性和显性模型中具有很强的相关性（TT 与 CC：OR=1.586，95%CI（1.131，2.225），=0.008；TT 与 TC/CC：OR=1.391，95%CI（1.073，1.804），=0.013）。TT 基因型的表达水平明显高于 CC 基因型（=0.012），且病例组的表达也高于对照组（=0.013）。
rs6489721 的纯合风险等位基因（TT）与增强的表达相关，导致中国人群中 NIHL 的发生。
