Álvarez-Suárez Alan Saúl, Dastager Syed G, Bogdanchikova Nina, Grande Daniel, Pestryakov Alexey, García-Ramos Juan Carlos, Pérez-González Graciela Lizeth, Juárez-Moreno Karla, Toledano-Magaña Yanis, Smolentseva Elena, Paz-González Juan Antonio, Popova Tatiana, Rachkovskaya Lyubov, Nimaev Vadim, Kotlyarova Anastasia, Korolev Maksim, Letyagin Andrey, Villarreal-Gómez Luis Jesús
Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, México. Blvd. Universitario #1000, Unidad Valle de las Palmas, Tijuana 22260, Baja California, México.
National Collection of Industrial Microorganisms (NCIM), CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
Micromachines (Basel). 2020 Apr 22;11(4):441. doi: 10.3390/mi11040441.
Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit™ silver nanoparticles (Ag-Si/AlO) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58-60 °C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria and Escherichia coli, Gram-positive and the fungus . The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-AlO-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-AlO-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity.
皮肤烧伤和溃疡因其高感染风险而被视为难以愈合的伤口。因此，设计新型伤口敷料的需求日益增长。这项工作的目的是研究通过静电纺丝制备的聚（ε-己内酯）/聚（乙烯基吡咯烷酮）（PCL/PVP）微纤维以及负载有Argovit™银纳米颗粒的吸附剂（Ag-Si/AlO）作为复合伤口敷料组成成分的性能。使用扫描电子显微镜（SEM）、差示扫描量热法（DSC）、傅里叶变换红外光谱（FTIR）和电感耦合等离子体发射光谱（ICP-OES）对纤维和吸附剂的物理化学性质进行了表征。还评估了纤维的力学性能。这项工作的结果表明，测试的纤维支架具有适合伤口敷料设计的熔点（58-60°C）。此外。它们在生理溶液中放置七天后仍显示稳定，在微观尺度上未因PVP释放而出现宏观损伤。粒径较大的造粒吸附剂显示出最佳的吸水能力。纤维和吸附剂对革兰氏阴性菌和大肠杆菌、革兰氏阳性菌以及真菌均表现出抗菌活性。PCL/PVP比例为85:15制备的支架具有最佳的物理化学性质，这种聚合物支架表现出最强的抗菌活性，且不影响人成纤维细胞的细胞活力。在所有测试的吸附剂中，造粒的Ag/Si-AlO-3吸附剂随时间推移具有最佳的吸水能力和较高的抗菌活性。在接下来的工作中，将使用PCL/PVP 85:15微纤维与选定的Ag/Si-AlO-3吸附剂的组合来制备具有渗出液保留、生物相容性和抗菌活性的伤口敷料。
