Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Program in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, 76010 Queretaro, Mexico; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228-230 ERML, 1201 W. Gregory Dr., Urbana, IL 61801, United States.
Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Program in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, 76010 Queretaro, Mexico.
Food Res Int. 2020 Jun;132:109097. doi: 10.1016/j.foodres.2020.109097. Epub 2020 Feb 11.
Ulcerative colitis (UC) is a condition that has been rising in the number of cases around the world. Food products made from natural ingredients such as corn and common bean might serve as alternatives for the treatment of UC. This study aimed to assess the anti-inflammatory effect of the consumption of a baked corn and bean snack (CBS) in an in vivo model of UC using 2% dextran sodium sulfate (DSS) as inductor of colitis. CD-1 mice (45, n = 9/group) were randomly separated into 5 groups, treated for 6-weeks as follows: G1 (basal diet, BD), G2 (2% DSS), G3 (20 g CBS/body weight BW/day + BD), G4 (40 g CBS/BW/day + BD) and G5 (60 g CBS/BW/day + BD). BW, Disease Activity Index (DAI), and feces were collected throughout the treatment. After euthanasia, organs (spleen, liver, and colon) were excised and weighed. Feces were analyzed for β-glucuronidase (β-GLUC) activity and gas-chromatography. The colons were analyzed for histopathology, myeloperoxidase (MPO) activity, and gene analysis. At the end of treatments, among the DSS-induced groups, G3 exhibited the lowest BW losses (11.5%), MPO activity (10.4%) and β-GLUC (8.6%). G4 presented the lowest DAI (0.88), relative spleen weight, and histological inflammation score (p < 0.05). Compared to G2, CBS consumption significantly (p < 0.05) reduced serum TNF-α, IL-10, and MCP-1 levels. The fecal metabolome analysis ranked 9-decenoic acid, decane, and butyric acid as the main contributors of pathways associated with the β-oxidation of fatty acids. G4 showed the highest fecal/cecal contents of short-chain fatty acids among all the DSS-induced groups. For the gene expression, G4 was clustered with G1, showing a differential inhibition of the pro-inflammatory genes Il1r1, Il1a, Tlr4, Tlr2, and Tnfrsf1b. In conclusion, CBS consumption decreased the inflammatory state and reduced the expression of the IL-1 receptor, TLR, and TNF-α-associated pathways in DSS-induced UC in CD-1 mice.
溃疡性结肠炎（UC）是一种在全球范围内发病率不断上升的疾病。以玉米和普通豆为原料制成的食品可能是治疗 UC 的替代方法。本研究旨在评估食用烘焙玉米豆零食（CBS）对 2%葡聚糖硫酸钠（DSS）诱导的 UC 体内模型的抗炎作用。将 45 只 CD-1 小鼠（n=9/组）随机分为 5 组，6 周内分别进行以下处理：G1（基础饮食，BD）、G2（2% DSS）、G3（20 g CBS/体重 BW/天+BD）、G4（40 g CBS/BW/天+BD）和 G5（60 g CBS/BW/天+BD）。整个治疗过程中收集 BW、疾病活动指数（DAI）和粪便。安乐死后，切除并称重器官（脾、肝和结肠）。分析粪便中的β-葡糖醛酸酶（β-GLUC）活性和气相色谱。分析结肠的组织病理学、髓过氧化物酶（MPO）活性和基因分析。在治疗结束时，在 DSS 诱导的各组中，G3 表现出最低的 BW 损失（11.5%）、MPO 活性（10.4%）和β-GLUC（8.6%）。G4 表现出最低的 DAI（0.88）、相对脾脏重量和组织学炎症评分（p<0.05）。与 G2 相比，CBS 消耗显著（p<0.05）降低了血清 TNF-α、IL-10 和 MCP-1 水平。粪便代谢组学分析将 9-癸烯酸、癸烷和丁酸列为与脂肪酸β-氧化相关途径的主要贡献者。在所有 DSS 诱导的组中，G4 显示出最高的粪便/盲肠短链脂肪酸含量。在基因表达方面，G4 与 G1 聚类，表现出对促炎基因 Il1r1、Il1a、Tlr4、Tlr2 和 Tnfrsf1b 的差异抑制。总之，CBS 消耗降低了 CD-1 小鼠 DSS 诱导的 UC 中的炎症状态，并降低了 IL-1 受体、TLR 和 TNF-α 相关途径的基因表达。
