微柱高效液相色谱法测定苦杏仁和桃仁中苦杏仁苷的含量
MicroHLPC determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae.
作者信息
He L Y, Li B M
机构信息
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
出版信息
Biomed Chromatogr. 1988 Nov;2(6):271-3. doi: 10.1002/bmc.1130020609.
The application of micro HPLC to the determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae is described. Amygdalin is separated at ambient temperature on a reversed phase column of U-Finepak SIL C18(150 x 0.5 mm) with methanol + water (25:75 v/v) as the mobile phase at a flow rate of 10 microL/min. The results are calculated by the internal standard method. The linear range is 1-7 micrograms. The CV and recovery of pure amygdalin are 1.47% (n = 10) and 98.13%, respectively. The results of analysis are lower than those obtained by TLC, but microHPLC is much simpler, faster, and more sensitive and reproducible than TLC.
描述了微型高效液相色谱法在测定苦杏仁和桃仁中苦杏仁苷的应用。苦杏仁苷在室温下于U-Finepak SIL C18（150×0.5 mm）反相柱上分离，以甲醇+水（25:75 v/v）为流动相，流速为10微升/分钟。结果采用内标法计算。线性范围为1 - 7微克。纯苦杏仁苷的变异系数（CV）和回收率分别为1.47%（n = 10）和98.13%。分析结果低于薄层色谱法（TLC）所得结果，但微型高效液相色谱法比薄层色谱法更简单、快速、灵敏且可重复。
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