Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China.
Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China; Key laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
Sci Total Environ. 2020 Jul 20;727:138708. doi: 10.1016/j.scitotenv.2020.138708. Epub 2020 Apr 16.
Antibiotic resistance genes (ARGs) harbored by plant microbiomes have been implicated as a potential risk to public health via food chain, especially directly edible fruits and vegetables. Here, we investigated the microbiome and antibiotic resistome in soil-strawberry ecosystem using shotgun metagenomic sequencing. The results showed that the enterobacterial population dominated the endophytes of strawberry fruits. Moreover, 85 subtypes of ARGs, including several clinically important ARGs, were detected in the strawberry fruit metagenomes. Additionally, host tracking analysis in combination with antibiotic-resistant bacterial isolate screening suggested that fruit-borne ARGs were mainly carried by members of the Enterobacteriaceae family. Unexpectedly, most of fruit-borne isolates were found to be resistant to several clinically important antimicrobials, e.g., erythromycin and cephalexin. Our findings provide broad insights into endophytic antibiotic resistomes of direct edible strawberry fruits and their potential hosts, and highlight the potential exposure risks of plant microbiomes to the human food chain.
植物微生物组中携带的抗生素耐药基因（ARGs）通过食物链被认为是对公众健康的潜在威胁，尤其是直接可食用的水果和蔬菜。在这里，我们使用高通量宏基因组测序技术研究了土壤-草莓生态系统中的微生物组和抗生素耐药组。结果表明，肠杆菌种群主导了草莓果实的内生菌。此外，在草莓果实宏基因组中检测到 85 种亚型的 ARGs，包括几种临床上重要的 ARGs。此外，宿主跟踪分析结合抗生素耐药细菌分离株筛选表明，水果携带的 ARGs 主要由肠杆菌科的成员携带。出乎意料的是，大多数水果携带的分离株被发现对几种临床上重要的抗生素，如红霉素和头孢氨苄具有耐药性。我们的研究结果为直接可食用的草莓果实及其潜在宿主的内生抗生素耐药组提供了广泛的见解，并强调了植物微生物组对人类食物链的潜在暴露风险。
