Li Ying, Cheng Lu, Xia Qian, Meng Maohua, Wang Qinying, Zeng Xiao, Jia Yuanyuan, Liu Chunying, Wu Lei, Chen Helin, Dong Qiang
Department of Prosthodontics, Stomatological Hospital of Guizhou Medical University Guiyang 550004, Guizhou, China.
College of Stomatology of Guizhou Medical University Guiyang 550004, Guizhou, China.
Am J Transl Res. 2023 Feb 15;15(2):1085-1096. eCollection 2023.
To explore the potential osteogenic induction mechanism of enamel matrix derivatives (EMDs) on bone marrow mesenchymal stem cell (BMSC) sheets with different titanium surface morphologies.
The BMSCs were inoculated on the surfaces of titanium alloys with different morphologies: anodic oxidation (AO), sand-blasted, large grit and acid-etched, and no treatment (control). The proliferation and osteogenic differentiation of BMSCs on the different surface morphologies were observed with the same concentration of EMDs. To further understand the osteogenic mechanism of EMDs on BMSC sheets with different morphologies, a real-time RT-PCR and a western blot were used to detect the overall levels of osteogenic genes and osteogenic proteins. Finally, to verify the osteogenic effect of BMSC sheets stimulated by EMDs in vivo, BMSC sheets with different morphologies were implanted into the subcutaneous tissue of the back of nude mice, and the bone formation was detected by HE staining.
The EMDs and surface morphology in the AO group synergically increased the expression levels of osteogenic active factors (RUNX2, OSX and OCN) and enhanced the osteogenic differentiation effect of BMSCs. The in vivo experiments showed that the BMSC sheets in the AO group were rich in osteogenic active factors, and promoted the formation of ectopic bone tissue after implantation into the subcutaneous tissue of the back of nude mice.
EMDs and AO morphology synergically enhance the secretion of bone osteogenic active factors of BMSCs and promote the formation of heterotopic bone.
探讨釉基质衍生物（EMDs）对不同钛表面形态的骨髓间充质干细胞（BMSC）片层的潜在成骨诱导机制。
将BMSCs接种于不同形态的钛合金表面：阳极氧化（AO）、喷砂、大颗粒酸蚀以及未处理（对照）。在相同浓度的EMDs作用下，观察BMSCs在不同表面形态上的增殖和成骨分化情况。为进一步了解EMDs对不同形态BMSC片层的成骨机制，采用实时RT-PCR和蛋白质免疫印迹法检测成骨基因和成骨蛋白的总体水平。最后，为验证EMDs刺激的BMSC片层在体内的成骨效果，将不同形态的BMSC片层植入裸鼠背部皮下组织，通过HE染色检测骨形成情况。
AO组中的EMDs和表面形态协同增加了成骨活性因子（RUNX2、OSX和OCN）的表达水平，并增强了BMSCs的成骨分化效果。体内实验表明，AO组的BMSC片层富含成骨活性因子，植入裸鼠背部皮下组织后促进了异位骨组织的形成。
EMDs和AO形态协同增强了BMSCs骨成骨活性因子的分泌，并促进了异位骨的形成。
