Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, Health Sciences, University of Utah, Salt Lake City, UT, United States.
Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, Health Sciences, University of Utah, Salt Lake City, UT, United States; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, United States.
Methods Cell Biol. 2020;157:143-167. doi: 10.1016/bs.mcb.2019.11.020. Epub 2020 Jan 21.
Three-dimensional (3D) reconstruction of highly functional tissues is of great importance in advancing the clinical benefit of tissue engineering and regenerative medicine. In the last quarter century, many studies have found that by engineering a 3D microenvironment that resembles the in vivo tissue condition, cells exhibit behaviors and functions that reflect those of native tissue. Biomaterial scaffolds are a central technology for providing 3D microenvironments in vitro, and, in conjunction with diverse design and cell seeding advents, have produced highly functional and complex 3D tissues. Here, we describe a new approach to creating 3D cell-dense tissue-like constructs without a biomaterial scaffold. Cell sheet technology with cell sheet layering strategies generates highly cell dense, engineered tissue capable of direct crosstalk with the tissue-engraftment surface, in addition to paracrine-mediated signaling. In this chapter, we will introduce methods of reconstructing 3D tissue using cell sheet technology and the advantages of a scaffold-free design.
三维（3D）重建高度功能性组织对于推进组织工程和再生医学的临床应用具有重要意义。在过去的四分之一个世纪中，许多研究发现，通过构建类似于体内组织条件的 3D 微环境，细胞表现出反映天然组织特性的行为和功能。生物材料支架是提供体外 3D 微环境的核心技术，并且与各种设计和细胞接种策略相结合，已经产生了高度功能性和复杂的 3D 组织。在这里，我们描述了一种无需生物材料支架即可创建 3D 细胞密集组织样结构的新方法。细胞片技术与细胞片分层策略相结合，可以生成高度密集的工程化组织，除了旁分泌介导的信号传递外，还可以与组织移植表面直接进行串扰。在本章中，我们将介绍使用细胞片技术重建 3D 组织的方法以及无支架设计的优势。
