Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, Republic of Korea.
The Center for Marine Integrated Biomedical Technology (BK21 PLUS), Pukyong National University, Busan, Republic of Korea.
Tissue Eng Regen Med. 2022 Oct;19(5):969-986. doi: 10.1007/s13770-022-00467-z. Epub 2022 Jul 20.
Various tissues, including the heart, cornea, bone, esophagus, bladder and liver, have been vascularized using the cell sheet technique. It overcomes the limitations of existing techniques by allowing small layers of the cell sheet to generate capillaries on their own, and it can also be used to vascularize tissue-engineered transplants. Cell sheets eliminate the need for traditional tissue engineering procedures such as isolated cell injections and scaffold-based technologies, which have limited applicability. While cell sheet engineering can eliminate many of the drawbacks, there are still a few challenges that need to be addressed. The number of cell sheets that can be layered without triggering core ischemia or hypoxia is limited. Even when scaffold-based technologies are disregarded, strategies to tackle this problem remain a substantial impediment to the efficient regeneration of thick, living three-dimensional cell sheets. In this review, we summarize the cell sheet technology in myocardial infarcted tissue regeneration.
各种组织，包括心脏、角膜、骨、食管、膀胱和肝脏，已经使用细胞片技术进行了血管化。它通过允许细胞片的小层自行生成毛细血管来克服现有技术的局限性，并且还可以用于血管化组织工程移植物。细胞片消除了传统组织工程程序的需要，例如孤立细胞注射和基于支架的技术，这些技术的适用性有限。虽然细胞片工程可以消除许多缺点，但仍有一些挑战需要解决。可以分层而不会引发核心缺血或缺氧的细胞片数量是有限的。即使不考虑基于支架的技术，解决这个问题的策略仍然是有效再生厚的、活的三维细胞片的主要障碍。在这篇综述中，我们总结了细胞片技术在心肌梗死组织再生中的应用。
