Potten C S, Roberts S A, Chwalinski S, Loeffler M, Paulus U
Paterson Institute for Cancer Research, Christie Hospital & Holt Radium Institute, Manchester, U.K.
Cell Tissue Kinet. 1988 Jul;21(4):231-46. doi: 10.1111/j.1365-2184.1988.tb00783.x.
Various counts have been made of the number of mitotic figures in whole crypts and sections of crypts of the small intestine of the mouse. Samples were analysed from animals killed at different times of the day and at different times after administration of vincristine. Measurements have been made of the size of mitotic and interphase nuclei and of the radial position of mitotic figures. The correction factor, f, which is required to take into account the enhancement of mitotic counts in sections as a consequence of their centripetal position has been investigated. The results indicate the following: (1) transverse sections of the crypt differ from longitudinal sections if they involve cutting the intestine before fixation which may result in a relaxation of the crypt and its widening by 25%; (2) columnar cell nuclei have a shape that resembles a sphere flattened so that the average diameter is 20% greater in crypt transverse sections; (3) mitotic nuclei tend to be about half-way between the crypt edge and the central axis of the crypt; (4) between about four and seven times more mitotic figures have their mitotic axis parallel to the long axis of the crypt; (5) about one-third of all mitotic figures in a crypt are seen in a longitudinal section of the crypt. If this is related to the number of cells in the crypt as a whole and in a section, a correction factor fD for the mitotic index of 0.59 is obtained; (6) the correction factor fT derived from the shape and position of the mitotic figures measured in 3 microns longitudinal sections is 0.53; (7) relating cell cycle and mitotic accumulation data using a computer-based model of the crypt also permits a correction factor fmod to be estimated. This gives a value of 0.66. When sectioned material is used to calculate a mitotic index the most appropriate correction factor is fD; for mouse small intestine it is 0.59.
对小鼠小肠全隐窝及隐窝切片中的有丝分裂细胞数量进行了多种计数。分析了在一天中不同时间以及给予长春新碱后不同时间处死的动物样本。测量了有丝分裂期和间期细胞核的大小以及有丝分裂细胞的径向位置。研究了校正因子f，该因子用于考虑由于切片的向心位置导致的有丝分裂计数增加。结果表明：（1）如果在固定前切割肠道，隐窝的横切面与纵切面不同，这可能导致隐窝松弛并使其增宽25%；（2）柱状细胞核的形状类似于扁平的球体，因此在隐窝横切面上平均直径大20%；（3）有丝分裂细胞核倾向于位于隐窝边缘与隐窝中心轴之间的大约中间位置；（4）有丝分裂轴与隐窝长轴平行的有丝分裂细胞数量大约多四到七倍；（5）隐窝中约三分之一的有丝分裂细胞出现在隐窝的纵切面上。如果这与整个隐窝和切片中的细胞数量相关，则有丝分裂指数的校正因子fD为0.59；（6）从3微米纵切面上测量的有丝分裂细胞的形状和位置得出的校正因子fT为0.53；（7）使用基于计算机的隐窝模型关联细胞周期和有丝分裂积累数据，也可以估计校正因子fmod。其值为0.66。当使用切片材料计算有丝分裂指数时最合适的校正因子是fD；对于小鼠小肠，它是0.59。
