使用抗体包被的红细胞进行抗原的灵敏检测以及用于带有表面免疫球蛋白细胞的玫瑰花结试验。
Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins.
作者信息
Ling N R, Bishop S
出版信息
J Immunol Methods. 1977;15(3):279-89. doi: 10.1016/0022-1759(77)90065-5.
PMID:323365
Abstract
Conditions for an improved chromic chloride method for the attachment of antibody to red cells are described. The method, which is applicable to whole Ig fractions as well as affinity-purified antibodies, is reproducible and has a sensitivity for detection of antigen in the nanogram range. The coated cells may be used in a rosette assay for the detection of cell surface-bound antigens.
摘要
本文描述了一种改良的氯化铬法，用于将抗体附着于红细胞的条件。该方法适用于完整的免疫球蛋白组分以及亲和纯化的抗体，具有可重复性，并且在纳克范围内对抗原检测具有敏感性。包被的细胞可用于玫瑰花结试验，以检测细胞表面结合的抗原。
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