Huang Qing, Liu Yuxia, Zhang Cuizhong, Zhang Zhenfa, Liu Fengping, Peng Jinyun
College of Chemistry and Chemical Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China.
College of Physics and Electronic Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China.
ACS Omega. 2020 Apr 9;5(15):8423-8431. doi: 10.1021/acsomega.9b02998. eCollection 2020 Apr 21.
Owing to the importance of epinephrine as a neurotransmitter and hormone, sensitive methods are required for its detection. We have developed a sensitive photoelectrochemical (PEC) microsensor based on gold quantum dots (Au QDs) decorated on a nickel tetraminophthalocyanine-graphene oxide (NiTAPc-Gr) composite. NiTAPc was covalently attached to the surface of graphene oxide to prepare NiTAPc-Gr, which exhibits remarkable stability and PEC performance. In situ growth of Au QDs on the NiTAPc-Gr surface was achieved using chemical reduction at room temperature. The synthesized materials were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, and electrochemical impedance spectroscopy. Au QDs@NiTAPc-Gr provided a much greater photocurrent than NiTAPc-Gr, making it suitable for the ultrasensitive PEC detection of epinephrine. The proposed PEC strategy exhibited a wide linear range of 0.12-243.9 nM with a low detection limit of 17.9 pM (S/N = 3). Additionally, the fabricated PEC sensor showed excellent sensitivity, remarkable stability, and good selectivity. This simple, fast, and low-cost strategy was successfully applied to the analysis of human serum samples, indicating the potential of this method for clinical detection applications.
由于肾上腺素作为神经递质和激素的重要性，需要灵敏的方法对其进行检测。我们基于修饰在四氨基酞菁镍-氧化石墨烯（NiTAPc-Gr）复合材料上的金量子点（Au QDs）开发了一种灵敏的光电化学（PEC）微传感器。将NiTAPc共价连接到氧化石墨烯表面以制备NiTAPc-Gr，其具有显著的稳定性和PEC性能。通过室温化学还原实现了在NiTAPc-Gr表面原位生长Au QDs。采用傅里叶变换红外光谱、紫外可见光谱、X射线光电子能谱、扫描电子显微镜、透射电子显微镜和电化学阻抗谱对合成材料进行了表征。Au QDs@NiTAPc-Gr比NiTAPc-Gr提供了更大的光电流，使其适用于肾上腺素的超灵敏PEC检测。所提出的PEC策略表现出0.12 - 243.9 nM的宽线性范围，检测限低至17.9 pM（S/N = 3）。此外，所制备的PEC传感器具有出色的灵敏度、显著的稳定性和良好的选择性。这种简单、快速且低成本的策略成功应用于人体血清样本分析，表明该方法在临床检测应用中的潜力。
