猕猴灵长类动物的淋巴组织免疫组化

Ormerod L D, Osborn K G, Lowenstine L J, Meyer P R, Parker J W, Smith R E, Taylor C R

Department of Ophthalmology, University of Southern California Medical Center, Los Angeles.

Clin Exp Immunol. 1988 Dec;74(3):435-42.

The immunohistochemical localizations of 53 monoclonal antibodies with specificities expressed in human lymphoid tissue were investigated on lymph node tissues from Macaca mulatta, M. speciosa, and M. fascicularis using immunoperoxidase techniques. Thirty antibodies showed comparative cross-reactivity. The micro-anatomic distribution of these antigens was closely homologous to that observed in man and antigen densities appeared to be similar. A useful monoclonal antibody panel for immunopathological investigations in macaques was, thereby, defined. Of several pan-T antibodies studied, only OKT11(CD2) showed cross-reactivity. A detailed analysis of the immunocyto-architecture of five rhesus lymph nodes was undertaken. Automated flow cytometry of M. mulatta and M. speciosa peripheral blood showed a broadly similar pattern to man.

利用免疫过氧化物酶技术，对恒河猴、食蟹猴和黑冠猕猴淋巴结组织进行研究，以探讨53种在人类淋巴组织中表达的具有特异性的单克隆抗体的免疫组织化学定位。30种抗体显示出比较性交叉反应。这些抗原的微观解剖分布与人观察到的情况密切同源，且抗原密度似乎相似。由此确定了一组用于猕猴免疫病理学研究的有用单克隆抗体。在所研究的几种泛T抗体中，只有OKT11（CD2）显示出交叉反应。对5个恒河猴淋巴结的免疫细胞结构进行了详细分析。恒河猴和食蟹猴外周血的自动流式细胞术显示出与人大致相似的模式。