Kap Yolanda S, van Meurs Marjan, van Driel Nikki, Koopman Gerrit, Melief Marie-Jose, Brok Herbert P M, Laman Jon D, 't Hart Bert A
Departments of Immunobiology, Biomedical Primate Research Center, Rijswijk, The Netherlands.
J Histochem Cytochem. 2009 Dec;57(12):1159-67. doi: 10.1369/jhc.2009.954123. Epub 2009 Sep 3.
Non-human primates (NHPs) offer valuable animal models for basic research into human diseases and for the preclinical validation of new therapeutics. Detailed in situ examination of the involved cell types using immunohistochemistry is often hampered by the lack of cross-reactive antibodies (Abs). In the current study, we have tested a large panel of monoclonal antibodies raised against human leukocyte differentiation and activation markers for cross-reactivity on cryosections of lymphoid tissue from six NHP species. In total, we have tested 130 Abs against 69 antigens expressed in tissues from one great ape species (chimpanzee/Pan troglodytes), two Old World species (rhesus macaque/Macaca mulatta and cynomolgus macaque/Macaca fascicularis), and three New World species (common marmoset/Callithrix jacchus, cotton-top tamarin/Saguinus oedipus, and owl monkey/Aotus triviogatus). We have found a large panel of cross-reactive Abs: 93 of 102 (91%) in chimpanzee, 97 of 125 (78%) in rhesus macaque, 70 of 109 (64%) in cynomolgus macaque, 69 of 116 (60%) in common marmoset, 40 of 81 (49%) in cotton-top tamarin, and 35 of 80 (44%) in owl monkey. The availability of a reliable panel of cross-reactive markers is important to gaining further insight into immunological processes in disease-affected tissues from NHP species.
非人灵长类动物（NHPs）为人类疾病的基础研究和新疗法的临床前验证提供了有价值的动物模型。使用免疫组织化学对所涉及的细胞类型进行详细的原位检查常常因缺乏交叉反应性抗体（Abs）而受阻。在本研究中，我们测试了一大组针对人类白细胞分化和活化标志物产生的单克隆抗体，以检测其在六种非人灵长类动物物种的淋巴组织冰冻切片上的交叉反应性。我们总共测试了130种抗体，针对一种大型猿类物种（黑猩猩/黑猩猩属）、两种旧世界物种（恒河猴/猕猴和食蟹猴/食蟹猕猴）以及三种新世界物种（普通狨猴/狨猴属、棉顶狨猴/绢毛猴属和夜猴/夜猴属）组织中表达的69种抗原。我们发现了一大组交叉反应性抗体：黑猩猩中102种中的93种（91%）、恒河猴中125种中的97种（78%）、食蟹猴中109种中的70种（64%）、普通狨猴中116种中的69种（60%）、棉顶狨猴中81种中的40种（49%）以及夜猴中80种中的35种（44%）。可靠的交叉反应性标志物组的可用性对于进一步深入了解非人灵长类动物物种受疾病影响组织中的免疫过程非常重要。
