Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Department of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Christchurch 7647, New Zealand; Riddet Research Institute, Palmerston North 4442, New Zealand.
Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Department of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Christchurch 7647, New Zealand; Riddet Research Institute, Palmerston North 4442, New Zealand.
Food Chem. 2020 Sep 15;324:126837. doi: 10.1016/j.foodchem.2020.126837. Epub 2020 Apr 20.
Evidences have shown that phytosome assemblies are novel drug delivery system. However, studies of phytosomes in food applications are scarce. The characteristics of milk phospholipid assemblies and their functionality in terms of in vitro digestibility and bioavailability of encapsulated nutrients (ascorbic acid and α-tocopherol) were studied. The phytosomes were fabricated using ethanolic evaporation technique. Spectral analysis revealed that polar parts of phospholipids formed hydrogen bonds with ascorbic acid hydroxyl groups, further, incorporating ascorbic acid or α-tocopherol into the phospholipid assembly changed the chemical conformation of the complexes. Phospholipid-ascorbic acid phytosomes yielded an optimal complexing index of 98.52 ± 0.03% at a molar ratio of 1:1. Phytosomes exhibited good biocompatibility on intestinal epithelial cells. The cellular uptake of ascorbic acid was 29.06 ± 1.18% for phytosomes. It was higher than that for liposomes (24.14 ± 0.60%) and for ascorbic acid aqueous solution (1.17 ± 0.70%).
有证据表明，植物药质体是一种新型的药物传递系统。然而，关于植物药质体在食品应用方面的研究还很少。本研究以牛奶磷脂为载体，采用醇浴蒸发法制备植物药质体，考察其对包埋物（抗坏血酸和α-生育酚）体外消化率和生物利用度的影响，同时对其结构特征和功能进行了研究。光谱分析表明，磷脂的极性部分与抗坏血酸的羟基形成氢键，进一步将抗坏血酸或α-生育酚包埋到磷脂组装体中，改变了复合物的化学构象。当磷脂与抗坏血酸的摩尔比为 1:1 时，复合物的最佳络合指数为 98.52 ± 0.03%。植物药质体对肠道上皮细胞表现出良好的生物相容性。与脂质体（24.14 ± 0.60%）和抗坏血酸水溶液（1.17 ± 0.70%）相比，植物药质体对抗坏血酸的细胞摄取率（29.06 ± 1.18%）更高。
