College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, PR China.
College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, PR China.
J Hazard Mater. 2020 Sep 5;396:122631. doi: 10.1016/j.jhazmat.2020.122631. Epub 2020 Apr 9.
A bacterial consortium for efficient decontamination of high-concentration Fe-Mn acid mine drainage (AMD) was successfully isolated. The removal efficiencies of Fe and Mn were effective, reaching 99.8 % and 98.6 %, respectively. High-throughput sequencing of the 16S rRNA genes demonstrated that the microbial community had changed substantially during the treatment. The Fe-Mn oxidizing bacteria Flavobacterium, Brevundimonas, Stenotrophomonas and Thermomonas became dominant genera, suggesting that they might play vital roles in Fe and Mn removal. Moreover, the pH of culture increased obviously after incubation, which was benefit for depositing Fe and Mn from AMD. The specific surface area of the biogenic Fe-Mn oxides was 108-121 m/g, and the surface contained reactive oxygen functional groups (-OH and -COOH), which also improved Fe and Mn removal efficiency. Thus, this study provides an alternative method to treat AMD containing high concentrations of Fe and Mn.
成功分离出一种用于高效净化高浓度铁锰酸性矿山废水（AMD）的细菌共生体。Fe 和 Mn 的去除效率均十分有效，分别达到 99.8%和 98.6%。16S rRNA 基因高通量测序表明，在处理过程中微生物群落发生了显著变化。Fe-Mn 氧化菌黄杆菌、短波单胞菌、寡养单胞菌和嗜热菌成为主要优势属，表明它们可能在 Fe 和 Mn 的去除中发挥重要作用。此外，培养后培养物的 pH 值明显升高，有利于从 AMD 中沉淀 Fe 和 Mn。生物成因的 Fe-Mn 氧化物的比表面积为 108-121 m/g，表面含有反应性氧官能团（-OH 和-COOH），这也提高了 Fe 和 Mn 的去除效率。因此，本研究为处理含有高浓度 Fe 和 Mn 的 AMD 提供了一种替代方法。
