National Poliovirus/Enterovirus Reference Laboratory, Hellenic Pasteur Institute, Athens, Greece; Diagnostic Department, Hellenic Pasteur Institute, Athens, Greece.
Diagnostic Department, Hellenic Pasteur Institute, Athens, Greece.
J Clin Virol. 2020 Jun;127:104349. doi: 10.1016/j.jcv.2020.104349. Epub 2020 Apr 21.
There are only sporadic data for the circulation of Enteroviruses (EVs) in Greece with previous studies reporting mainly the presence of Echoviruses (E) and Coxsackie viruses (CV) B.
We carried out a surveillance study for the molecular characterization of EVs detected in hospitalized patients throughout Greece as well as a phylogenetic analysis of the most frequently encountered serotypes.
Stools, cerebrospinal fluids, throat swabs and blood samples were collected from hospitalized patients with suspicion of EV infection. All samples were tested for EVs by rRT-PCR targeting the 5' untranslated region of EV genome. For positive samples, PCR amplification and sequencing targeting a part of VP1 region was performed.
We examined 831 samples and 209 were positive for EVs with Enterovirus B species being the most frequently amplified. E30, CVB5 and E9 were the most frequent serotypes of Enterovirus B species, whereas CVA6 and EV-A71 the most frequent serotypes of Enterovirus A species. Evs were significantly detected more frequently in stool samples compared to other types of specimens. Phylogenetic analysis revealed that most EV-A71 strains clustered in the subgenogroups C2 whereas all the CVA6 strains belonged to sub-genotype D3. Additionally, two different lineages of E30 and three different clusters of E9 viruses circulated simultaneously in Greece. Our data indicated that most EV strains from Greece were similar to strains circulating throughout Europe during the same period.
We provide a comprehensive picture of EVs circulating in Greece which can be helpful to interpret trends in EV diseases by associating them with circulating serotypes.
希腊仅有零星的肠病毒（EVs）循环数据，先前的研究主要报告了肠疹病毒（E）和柯萨奇病毒（CV）B 的存在。
我们对在希腊住院的患者中检测到的 EVs 进行了分子特征监测研究，并对最常见的血清型进行了系统发育分析。
从疑似 EV 感染的住院患者中采集粪便、脑脊液、咽拭子和血液样本。所有样本均通过针对 EV 基因组 5'非翻译区的 rRT-PCR 进行 EVs 检测。对于阳性样本，进行针对 VP1 区域一部分的 PCR 扩增和测序。
我们检查了 831 个样本，其中 209 个样本对 EVs 呈阳性，肠病毒 B 种是最常扩增的。E30、CVB5 和 E9 是肠病毒 B 种的最常见血清型，而 CVA6 和 EV-A71 是肠病毒 A 种的最常见血清型。与其他类型的标本相比，EVs 在粪便标本中的检出率明显更高。系统发育分析显示，大多数 EV-A71 株聚集在亚属 C2 中，而所有 CVA6 株均属于亚基因型 D3。此外，E30 有两种不同的谱系和 E9 病毒有三个不同的簇在希腊同时传播。我们的数据表明，来自希腊的大多数 EV 株与同期欧洲流行的株相似。
我们提供了希腊流行的 EVs 全面情况，通过将其与流行的血清型相关联，可以帮助解释 EV 疾病的趋势。
