Centro deMetabolómica y Bioanálisis, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, Boadilla del Monte, 28660 Madrid, Spain.
Department of Biopharmaceutics and Pharmacodynamics, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdańsk, Poland.
Nutrients. 2020 Apr 23;12(4):1188. doi: 10.3390/nu12041188.
Very preterm infants (VPI, born at or before 32 weeks of gestation) are at risk of adverse health outcomes, from which they might be partially protected with appropriate postnatal nutrition and growth. Metabolic processes or biochemical markers associated to extrauterine growth restriction (EUGR) have not been identified. We applied untargeted metabolomics to plasma samples of VPI with adequate weight for gestational age at birth and with different growth trajectories (29 well-grown, 22 EUGR) at the time of hospital discharge. A multivariate analysis showed significantly higher levels of amino-acids in well-grown patients. Other metabolites were also identified as statistically significant in the comparison between groups. Relevant differences (with corrections for multiple comparison) were found in levels of glycerophospholipids, sphingolipids and other lipids. Levels of many of the biochemical species decreased progressively as the level of growth restriction increased in severity. In conclusion, an untargeted metabolomic approach uncovered previously unknown differences in the levels of a range of plasma metabolites between well grown and EUGR infants at the time of discharge. Our findings open speculation about pathways involved in growth failure in preterm infants and the long-term relevance of this metabolic differences, as well as helping in the definition of potential biomarkers.
极早产儿（胎龄在 32 周或以下出生）存在健康状况不佳的风险，通过适当的产后营养和生长可以部分保护他们免受这种风险。尚未确定与宫外生长受限（EUGR）相关的代谢过程或生化标志物。我们对出生时具有适当胎龄体重且在出院时具有不同生长轨迹（29 例生长良好，22 例 EUGR）的极早产儿的血浆样本应用了非靶向代谢组学分析。多变量分析显示，生长良好的患者的氨基酸水平明显更高。在组间比较中，还确定了其他代谢物具有统计学意义。在甘油磷脂、鞘脂和其他脂质的水平方面发现了相关的差异（经多次比较校正）。随着生长受限严重程度的增加，许多生化物质的水平逐渐降低。总之，非靶向代谢组学方法揭示了在出院时生长良好和 EUGR 婴儿之间一系列血浆代谢物水平的先前未知差异。我们的研究结果推测了与早产儿生长失败相关的途径以及这种代谢差异的长期相关性，并有助于定义潜在的生物标志物。
